Karolina Kalbarczyk Email & Phone Number

San Jose, CA, US

Pleasanton, California, United States

• Technical lead on a key project, which included responsibility for project timelines, client communications, and technical planning to achieve target deadlines and required titer improvements for product delivery

Pleasanton, California, United States

(Continued employment after acquisition of Sestina Bio)

Pleasanton, California, United States

• Genetic engineering of strains for optimized biosynthesis of novel compounds
• Incorporated literature precedent and homology searches to build and balance multiple genetic pathways, which were further optimized by improving key bottlenecking enzymes with rational and empirical methods
• Identified and established various pipeline improvements by designing different scripting solutions and optimized workflows to enable robust high-throughput workflows(Acquired by Inscripta)

Emeryville, CA

• Metabolic and microbial engineering of strains for various applications in bioproduction
• Implemented python, JMP and SQL scripting for high throughput DNA and primer designs for new strain engineering
• Built and tested strains in high throughput using Tecan, Echo, and Bravo automation platforms
• Led collaborations between different departments to make timeline projections and execute on experiments to meet deliverables(Acquired by Ginkgo Bioworks)

Albany, New York Area

• Engineering synthetic communities for the production of biofuels and pharmaceuticals
• Project focused on designing microbial systems with interchangeable modules, with the first module dedicated to cellulose degradation and the second module specialized for bioproduct synthesis
• Research project combining the work of Dr. Mattheos Koffas in glucose to bioproduct synthesis with the work of Dr. Cynthia Collins in interspecies commutations systems
• Design and perform experiments in the pursuit of my PhD in Chemical and Biological Engineering

Troy, New York

• Teaching assistant for Biotechnology and Bioprocessing Laboratory course (CHME 4170) taught by Dr. Cynthia Collins
• Laboratory course for seniors in chemical engineering to fulfill lab requirement, teaching bioprocessing lab techniques including DNA cloning, gel electrophoresis, bacterial transformation, bacterial cell culture.
• TA responsibilities included running and troubleshooting experiments ahead of time, preparation of materials for weekly labs, grading prelabs and worksheets, instructing and supervising lab class, and grading final.

Troy, New York

• Teaching assistant for Biochemical Engineering course (CHME 4430) taught by Dr. Cynthia Collins
• Elective course for juniors and seniors in chemical engineering, teaching biochemical engineering topics including biochemistry, enzyme kinetics, cell growth, bioreactors, sterilization, downstream processing, and.

Greater Los Angeles Area

• Focused on performing experiments of direct evolution using Chemical and Biomolecular Engineering
• Basic lab work revolving around creating libraries, Purpald and TMB assays, and gas chromatography to select for particular stereochemistry

Greater Los Angeles Area

• Focused on performing directed evolution of a range of terpene synthases to cyclize non-natural substrates and improve efficiency of cyclization of natural substrates

Greater Los Angeles Area

• Worked on the directed evolution of terpene synthases for non-natural C15 substrates
• Focused on performing experiments of directed evolution with high-throughput screening assays to develop mutant terpene synthases to cyclize non-natural substrates into new products, with future potential for.

Greater Los Angeles Area

• Worked on the directed evolution of cytochrome P450 for enantioselective meso-desymmetrization
• Focused on performing experiments of directed evolution with high-throughput screening assays to develop higher enantioselectivity P450 mutatnts

Urbana-Champaign, Illinois Area

• Worked on engineering a biochemical metabolic pathway of genes into E.coli and yeast cells
• Wanted a system where a variety of genes, primarily from Arabidopsis thaliana, were inserted into bacterial plasmids; the bacterial cells take an initial substrate and the enzymes corresponding to the genes react in.
• Focused heavily on work with P450 monooxygenases

Urbana-Champaign, Illinois Area

• Learned advanced calculus in combination with Mathematica to find generalized solutions for the two string problem, revolving around the situation of a string of two densities plucked at some initial function, and left.
• Developed general theory to use two dimensional oscillation and, expanding this to three dimensional oscillation, to make a mechanism to use the understanding of reflected wave patterns to test the integrity of a.
• Presented about uses of Mathematica in solving the two string problem at the International Mathematica Users Conference in 2008 at Wolfram Research Center in Champaign, IL

Urbana-Champaign, Illinois Area

• Summer research internship sponsored by the American Cancer Society, focused on cutting edge cancer research
• Performed experiments in a biochemistry laboratory with E.coli, DNA, and proteins, learning procedures for DNA gene insertion and modification
• Researched the potential use of p54 gene insertion to fight cancer, learning how to insert specific genes into a strand of DNA.

Doctor Of Philosophy (Phd), Chemical And Biological Engineering

My research is focused on combining the work of Dr. Mattheos Koffas in glucose to fatty acid production with the work of Dr. Cynthia.

B.S., Chemical Engineering And Premed

Activities and Societies: Tour Guide for Admissions Office, Research AssistantFocused in Biomolecular Research