Karyn Van De Mark Email & Phone Number

Rahway, New Jersey, US

Rahway, New Jersey, US

Providing cellular/ molecular biology expertise to early discovery programs in autoimmunity & immuno-oncology for the purpose of developing cellular assays for target validation and screening of small molecules/ biologics.

Cambridge, MA, US

• Working as part of the core functional Mammalian Expression subgroup within the Cellular Engineering Department of Drug Discovery:
• Developed and utilized multiple stable cell lines for purposes that include target validation, mechanism of action studies, signal transduction analysis, in addition to developing cellular assays for readout.
• To support crystallography and compound screening efforts, developed cell lines for recombinant protein production and scaled up to large scale culture then delivered product to functional groups for purification. o.
• Provided signal transduction analysis of protein kinase targets, and their signaling partners, for the purpose of studying the MOA of small molecule kinase inhibitors.
• Working closely with the Lead Scientist responsible for the B-Cell Cloning Initiative, expressed VH and VL combinations for positive pairings, and delivered functional antibody candidates to the project teams.
• Analyzed and presented all data at team and departmental meetings, in addition to writing monthly reports.

Cambridge, MA, US

• Developed stable cell lines and cellular reporter assays to use in high-throughput screening of small molecule inhibitors of a protein kinase.
• Calculated the corresponding IC50s of the inhibitory small molecules, and reported findings to Compound Profiling, and Chemists to assist in the drug discovery Structure Activity Relationship (SAR) process.
• Designed and conducted experiments to investigate the mechanism of action for protein kinase targets on their signal transduction cascade.
• Characterizated Morphosys and Dyax Fabs for different project teams.
• Screened 280,000 clones of a Human Brain cDNA library to identify a potentially new receptor for a ligand of interest.
• Developed stable cell lines expressing an ecotropic receptor for use in expression cloning by retroviral vectors.

Boston, MA, US

• Discovered an SFCA derivative with anti-tumor activity, and its potential mechanism of action.
• Participant of a retroviral project team using molecular techniques to study the transactivation mechanism of various retroviruses in relation to cancer.
• Partially purified a factor from serum linked to tumor immuno-suppression using protein purification techniques (M.A. Thesis).
• Assisted in the identification of a butyrate analog effective in the treatment of sickle cell anemia, and beta thalassemia, and analyzed samples obtained from patients undergoing treatment with butyrate to verify.
• Developed and implemented a lab manual of biochemical techniques, and safety procedures.
• Trained and supervised all laboratory aids and graduate students in laboratory techniques, and safety.Teaching Assistant-Biochemistry, Biomedical Laboratory and Clinical Sciences Program/MET 1995-1996

Boston, MA, US

• Participated in a project examining the down-regulation of class I MHC expression in oncogenically transformed cells and demonstrated that serum was involved in this phenomenon.
• Studied the molecular mechanisms of beta interferon in fibroblasts and demonstrated the dissociation of the anti-viral state from the anti-proliferative effects previously associated with this cytokine.
• Created expression vectors containing the beta and gamma globin gene promoters and applied this system to analyze the effects of butyrate in the treatment of sickle cell anemia at the cellular level.PUBLICATIONSVan De.

M.A., Pharmacology (Molecular)

Bs, Biotechnology