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I am a highly skilled, detail-oriented, results driven Scientist, and classically trained Cell/Molecular Biologist, with more than 25 years of drug discovery research experience. My focus is on the discovery and identification of small molecules and biologics to treat disease, in therapeutic areas that include Oncology, Autoimmune Disease, Inflammation, and Hemoglobinopathies. I am skilled in the design, execution and analysis of in vitro experiments for target identification/ validation and mechanism of action studies, as well as in large scale protein expression/production for crystallography, and reagent support.With my strong analytical and problem solving skills, the ability to work on multiple projects simultaneously, along with excellent interpersonal and collaborative skills, I have a strong reputation as being a vital contributor to project teams.Specialties:Macrophage Polarization StudiesCytokine AnalysisCellular imagingCell Line DevelopmentGene ExpressionSignal TransductionCell Cycle AnalysisRNAiCRISPR TechnologyLentiviral TransductionProtein Kinases
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Senior Scientist: Discovery ImmunologyMerck May 2022 - PresentRahway, New Jersey, Us -
Research Scientist: Immuno-Oncology Discovery BiologyMerck Mar 2014 - May 2022Rahway, New Jersey, UsProviding cellular/ molecular biology expertise to early discovery programs in autoimmunity & immuno-oncology for the purpose of developing cellular assays for target validation and screening of small molecules/ biologics.
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Senior Associate ScientistBiogen Idec 2006 - 2012Cambridge, Ma, UsWorking as part of the core functional Mammalian Expression subgroup within the Cellular Engineering Department of Drug Discovery:• Developed and utilized multiple stable cell lines for purposes that include target validation, mechanism of action studies, signal transduction analysis, in addition to developing cellular assays for readout.• To support crystallography and compound screening efforts, developed cell lines for recombinant protein production and scaled up to large scale culture then delivered product to functional groups for purification. o Utilized methods of n-glycosylation inhibition for the final product that resulted in successful crystal formation.• Provided signal transduction analysis of protein kinase targets, and their signaling partners, for the purpose of studying the MOA of small molecule kinase inhibitors.• Working closely with the Lead Scientist responsible for the B-Cell Cloning Initiative, expressed VH and VL combinations for positive pairings, and delivered functional antibody candidates to the project teams.• Analyzed and presented all data at team and departmental meetings, in addition to writing monthly reports. -
Associate Scientist IiiBiogen Idec 2001 - 2006Cambridge, Ma, Us• Developed stable cell lines and cellular reporter assays to use in high-throughput screening of small molecule inhibitors of a protein kinase.• Calculated the corresponding IC50s of the inhibitory small molecules, and reported findings to Compound Profiling, and Chemists to assist in the drug discovery Structure Activity Relationship (SAR) process.• Designed and conducted experiments to investigate the mechanism of action for protein kinase targets on their signal transduction cascade.• Characterizated Morphosys and Dyax Fabs for different project teams. • Screened 280,000 clones of a Human Brain cDNA library to identify a potentially new receptor for a ligand of interest.• Developed stable cell lines expressing an ecotropic receptor for use in expression cloning by retroviral vectors.• Presented results in written technical reports, and oral presentations at internal and departmental meetings.
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Senior Research Technician, Laboratory ManagerBoston University School Of Medicine- Cancer Research Center 1992 - 2001Boston, Ma, Us• Discovered an SFCA derivative with anti-tumor activity, and its potential mechanism of action.• Participant of a retroviral project team using molecular techniques to study the transactivation mechanism of various retroviruses in relation to cancer.• Partially purified a factor from serum linked to tumor immuno-suppression using protein purification techniques (M.A. Thesis).• Assisted in the identification of a butyrate analog effective in the treatment of sickle cell anemia, and beta thalassemia, and analyzed samples obtained from patients undergoing treatment with butyrate to verify effectiveness of the drug.• Developed and implemented a lab manual of biochemical techniques, and safety procedures.• Trained and supervised all laboratory aids and graduate students in laboratory techniques, and safety.Teaching Assistant-Biochemistry, Biomedical Laboratory and Clinical Sciences Program/MET 1995-1996 -
Research TechnicianDana-Farber Cancer Institute 1987 - 1992Boston, Ma, Us• Participated in a project examining the down-regulation of class I MHC expression in oncogenically transformed cells and demonstrated that serum was involved in this phenomenon.• Studied the molecular mechanisms of beta interferon in fibroblasts and demonstrated the dissociation of the anti-viral state from the anti-proliferative effects previously associated with this cytokine.• Created expression vectors containing the beta and gamma globin gene promoters and applied this system to analyze the effects of butyrate in the treatment of sickle cell anemia at the cellular level.PUBLICATIONSVan De Mark K, Chen JS, Steliou K, Perrine SP, Faller DV., Alpha-lipoic acid induces p27Kip-dependent cell cycle arrest in non-transformed cell lines and apoptosis in tumor cell lines.J Cell Physiol., 2003; 194 (3): 325-340 Boosalis, M. Bandyopadhyay, R. Bresnick, E., Pace, B., Van De Mark, K., Zhang, B., Faller, D., Perrine, S., Short chain fatty acid derivatives stimulate cell proliferation and induce STAT-5 activation.Blood, 2001; 97(10): 3259-3267Choi, S., Van De Mark, K., Faller, D., Identification of a cis-acting element in the class I Major Histocompatability Complex gene promoter responsive to activation by retroviral sequences Journal of Virology, 1997; 71 (2): 965-970.Mundschau, L, Van De Mark, K., Faller, D., Beta-interferon resistance in Balb/C-3T3 fibroblasts: dissociation of anti-viral and anti-proliferative activities of beta-interferon.Cancer Molecular Biology, 1994; 1 (1):107-129Koka, P., Van De Mark, K., Faller, D., Transactivation of genes encoding activation-associated Human T lymphocyte surface protein by murine retroviral sequences. Journal of Immunology, 1991; 146 (7): 2417-2425PATENTGorelik, L., Baker, D.P, Hession, C.A., Van De Mark, K., Arduini, R.M., Wildes, C., Antibodies to Phosphorylated IRAK4. US Patent Application: 20100279316, filed November 4, 2010.
Karyn Van De Mark Skills
Karyn Van De Mark Education Details
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Boston University School Of MedicinePharmacology (Molecular) -
Worcester Polytechnic InstituteBiotechnology
Frequently Asked Questions about Karyn Van De Mark
What company does Karyn Van De Mark work for?
Karyn Van De Mark works for Merck
What is Karyn Van De Mark's role at the current company?
Karyn Van De Mark's current role is Senior Scientist at Merck.
What is Karyn Van De Mark's email address?
Karyn Van De Mark's email address is ka****@****wpi.edu
What is Karyn Van De Mark's direct phone number?
Karyn Van De Mark's direct phone number is (617) 914*****
What schools did Karyn Van De Mark attend?
Karyn Van De Mark attended Boston University School Of Medicine, Worcester Polytechnic Institute.
What skills is Karyn Van De Mark known for?
Karyn Van De Mark has skills like Signal Transduction, Mammalian Cell Culture, Protein Expression, Rnai, Assay Development, Molecular Biology, Rt Pcr, Biochemistry, Drug Discovery, Cell Signaling, Recombinant Dna Technology, Elisa.
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