Katharine Wright Email & Phone Number

Plymouth Meeting, Pa, Us

Structural basis of recognition of T cell engagers that target MHC-neoantigens. - Produced and purified bispecific and full-length antibodies to be used as immuno-therapeutics for solid tumors (antibodies for MHC-p53R175H, KRASG12V, and IDH2R140Q). - Complex structures of antibodies with their MHC-neoantigen counterparts using X-ray crystallography (p53R175H, IDH2R140Q) and cryoEM (KRASG12V). - Biophysical characterization of MHC-neoantigen/antibody by SPR and thermal shift. - Determined rules of engagement for potential ‘off-the-shelf’ therapeutics. - Structural-guided affinity maturation through protein engineering. - Point of contact for CROs (molecular biology, DNA production)Regulation of the HECT E3 ligase, NEDD4L. - Determined the regulatory mechanism of NEDD4L through various biochemical in vitro and fluorescence-based binding assays. - NEDD4L inhibition studies using repurposed drugs. - Investigated the mechanism of regulation of voltage-gated sodium channels (NaVs) by NEDD4L with emphasis on protein:protein interactions, co-crystallization and in vitro assays. - Determined sites of ubiquitination of NaV1.7 by MS/MS.Trained and mentored: 1 current graduate student (Sara Nathan); 1 undergraduate student (Wendy Xia); 2 post-baccalaureate fellows (P. Aitana Azurmendi, Kim Phan); and 3 graduate rotation students.

Mechanism of MID1, a ubiquitin RING E3 ligase. - Synthesized, purified and characterized MID1 proteins using chromatography methods, gel electrophoresis, chemiluminescent assays and dynamic light scattering. - Analyzed the effect of XLOS-observed mutations on MID1 protein structure using multi-dimensional (2D) Nuclear Magnetic Resonance (NMR). - Performed 3D NMR to solve the tertiary structure of the microtubule-associated domain of MID1. - Analyzed protein-protein interactions using numerous methods: chemical shift perturbation 2D NMR, gel filtration and native gel electrophoresis.Trained and mentored: 1 graduate, 4 undergraduate and 3 high school students.Commissioned all the cataloging and acquiring of new instruments and chemicals for the lab.

New York, New York, Us

Worked in the Instrumental/Analytical department running samples and maintaining the HPLC's and GC's. Was tasked with cataloging all spectra into IFF database.

Brussels, Be

Worked every summer during undergraduate studies at Solvay (formerly known as Rhodia Inc). In 2011, I worked in the Agricultural & Development department investigating potential pesticide candidates for best drift control benefits and documented all safety precautions and managed numerous reactions. In 2009-2019, I worked in the R&D departments distinguishing between different polymers and additives to improve results of chemical reactions as well as collaborating with fellow employees and communicated hypothesis, procedures and findings to R&D team. In 2008, I worked in the Analytical & Technology department validating wet chemistry methods.

Doctor Of Philosophy - Phd, Biochemistry

Bachelor Of Science (B.S.), Chemistry