Kathleen Jacque Email & Phone Number

• Multi-year experience in forensic DNA analysis and serology testing for blood, semen, saliva, urine, and feces. Proficient in the following: - Manual organic and paramagnetic nucleic acid extraction - Tecan liquid handlers - qPCR via 7500 Real-Time PCR system - PCR via 9700 & ProFlex thermal cyclers - Capillary electrophoresis via 3130xl & 3500xl - Data interpretation via GeneMapper™ ID-X - Quantifiler™ Duo & Trio DNA Quantification Kits - GlobalFiler™ & AmpFLSTR™ Identifiler™ Plus PCR amplification kits - Laboratory Information Management System (LIMS)• Extensive experience in a high throughput laboratory due to primary job of manual organic DNA extraction, qPCR quantitation, PCR amplification, and capillary electrophoresis. Manually extract 24 samples in a batch, extract multiple batches per week, and quantitate, amplify, and separate up to 80 samples at a time via Tecan liquid handlers. • Demonstrates continual desire to learn and aptitude by being 1 of 5 workers that over time has become proficient in every step of the autosomal DNA analysis process• Capitalizes on being detail oriented by completing ANAB internal auditor training and participating in internal audits using ISO/IEC 17025:2017 and AR 3125 • Willing to help where ever needed and be a team player. Demonstrated by becoming the interim lab technician for two years where valuable skills were acquired, such as contacting vendors, creating purchase orders, ordering and receiving supplies, reagent preparation, inventory, hazardous waste collection, assisting in internal and external audits, and implementing quality assurance and quality control guidelines. • Able to break down complex scientific conclusions when testifying in trial as an expert witness• Has been rated as exceeding expectations in teamwork and cooperation for the past 4 years in annual performance reviews

Huntington, Wv

• Completed inventory and restocked laboratory supplies weekly • Audited reagents monthly and organized the reagent log• Shadowed forensic DNA analysts in performing serology, extractions, quantitations, and amplifications• Participated in internal audit of the laboratory by showing auditor reagent logs, certificates of calibrations, validations, cleaning logs, preventative maintenance logs, and sitting in on the review of casework and personnel binders• Assisted in drafting analytical procedures for the Qiagen® Investigator™ Quantiplex HYres Kit

Palmer Park, Md

• Validated the YFiler® amplification chemistry kit on a 3130 Genetic Analyzer • Performed the following internal validation studies: threshold, sensitivity, contamination, precision, mixtures, concordance, and reproducibility• Experienced gained in the EZ1® DNA Investigator Kit, Quantifiler® Human, YFiler®, and GeneMapper® ID v3.2.1

Huntington, West Virginia Area

• Maintained cleanliness and working conditions of DNA training laboratory weekly • Prepared DNA training sessions for professional analysts• Shadowed forensic DNA analysts in performing extractions, quantitations, and amplifications

U.S. Navy Yard

• Researched and analyzed primary and secondary sources to write biographies of select U.S. warships that played key roles during the War of 1812 for an online dictionary• Mastered research, organization and analytical skills necessary to obtain successful results

Cmu

• Researched the effects of anti-oxidants on free radicals through protein experiments • Interpreted data and predicted future outcomes of results and experiments• Presented research progress to lab group regularly and collaborated to solve problems efficiently

Ms, Forensic Science, 4.0

Activities and Societies: MUFIA (Masters United Forensic Identification Association), Phi Kappa PhiDNA Analysis and Crime Scene.

Bs, Biomedical Science, History, 3.65

Activities and Societies: Alpha Sigma Tau, Panhellenic Council, Girls on the Run, Order of Omega, Rho LambdaMinor concentrations.