Kan Chantranuvatana Email & Phone Number

Redmond, Washington, United States

Redmond, Washington, United States

Seattle, Washington, United States

• Increased the amount of protein-oligo conjugation to 700-800 per year while maintaining below 5% failure rate• Aided in the development of multiplex plate format cysteine conjugation utilizing an automated liquid handler that allows up to 96 different antibodies and oligos to be labeled simultaneously• Provided support to process development and manufacturing by aiding in document revisions, troubleshooting failed processes, and optimizing procedures• Analyzed digital spatial profile data in R programming to investigate the binding of different proteins to various cell types on proprietary Nanostring instruments

Greater Seattle Area

• Production of protein-oligonucleotide conjugates by modification of the cysteine residues or the carbohydrate backbone; averaging approximately 400-500 conjugates per year. Purification, characterization, and contamination QC of conjugates performed using a combination of HPLC, spectrophotometer, GeoMx Digital Spatial Profiler, gel electrophoresis, and Next Gen Sequencing• Side research projects included guard banding conjugation conditions, streamlining of QC methods, optimization of conjugation methods, and maintenance of HPLC instrument

Boulder, Co

• Functional QC - Solution Affinity assay testing the binding Kd of oligos to protein using radioisotope labelling: HIS-Tagged and Zorbax protein binding assays• Design and test 80-100bp oligos in qPCR for optimal pairing and formation of primer-dimer• Gel Electrophoresis on PCR products and for biotin shift assays• Organizing and keeping inventory of multiple crucial reagents for other research departments.• Quantification of protein concentrations using nano-orange.

Boulder, Co

• Semi-Automated protein binding assay utilizing the TECAN robotic instrument• Use of a spectrophotometer to quantify and correctly adjust oligo concentrations• GDP and GLP laboratory environment

Centennial, Co

• Automated and Semi-Automated testing instruments.o Immucor – NEO for CMV, TPHA, ABO, and RBC pooled cells testso Novartis Diagnostics – Procleix TIGRIS for HIV, HBV, HCV, and WNV testso Bio-Rad – D10 for Hemoglobin A1c testingo Qiagen – EZ1 Advanced DNA isolationo Life Technologies – Viia7 Real Time PCR• Manual testingo Viral Slides and Viral EIAs used to detect a multitude of sexually transmitted diseases in donors.o Manual ABO/Rh typing and Hemoglobin S screeningo ASI RPR cards used to detect Syphilis• Involved in writing new protocols and regulations per FDA and CAP standards.

• Facilitating collaborative learning and designed study skills for students enrolled in historically challenging courses.• Developed training sessions for other Tutors and Supplemental Instruction Facilitators.• Weekly scheduled tutoring for students in all classes of Chemistry.• Creating mock exams and quizzes tailored to student needs.

• Conducted research involving protein binding affinity of different Synaptotagmin isoforms to a lipid bilayer.• Specific research involved identifying the diffusion coefficient of Synaptotagmin 7 laterally diffusing across a DOPC/DOPS lipid bilayer.• Designed and performed cell cultures, PCR, gel electrophoresis, purification and identification of proteins from E. coli.• Create new protocols for TIRF microscope. Performed tests utilizing a stopped-flow and spectrophotometer.• Presented findings at the annual Undergraduate Research Opportunity Program (UROP) symposium and local ACS convention

• Laboratory instructor for the following teaching labs:Inorganic Chemistry (Spring 2009, Spring 2010)Physical Chemistry I (Spring 2010)Physical Chemistry II (Fall 2011)Honors General Chemistry I (Fall 2011, Fall 2012)Honors General Chemistry II (Spring 2012)Biochemistry (Spring 2012, Spring 2013)• Performed cell cultures, PCR, protein and DNA gel electrophoresis, TIRF microscopy and FRET experiments for students and material analysis using MS, IR, HPLC, FPLC, FT-IR, and Spectrophotometer machines

Master Of Science (M.S.), Chemistry

Thesis Title: Single-Molecule Analysis of Synaptotagmin 7 Lateral Diffusion across a Supported Lipid Bilayer. Research focused.

Bachelor Of Science (B.S.), Chemistry

Activities and Societies: Golden Key, American Chemical Society