Kan Chantranuvatana Email & Phone Number

Redmond, Washington, United States

Redmond, Washington, United States

Seattle, Washington, United States

• Increased the amount of protein-oligo conjugation to 700-800 per year while maintaining below 5% failure rate
• Aided in the development of multiplex plate format cysteine conjugation utilizing an automated liquid handler that allows up to 96 different antibodies and oligos to be labeled simultaneously
• Provided support to process development and manufacturing by aiding in document revisions, troubleshooting failed processes, and optimizing procedures
• Analyzed digital spatial profile data in R programming to investigate the binding of different proteins to various cell types on proprietary Nanostring instruments

Greater Seattle Area

• Production of protein-oligonucleotide conjugates by modification of the cysteine residues or the carbohydrate backbone; averaging approximately 400-500 conjugates per year. Purification, characterization, and.
• Side research projects included guard banding conjugation conditions, streamlining of QC methods, optimization of conjugation methods, and maintenance of HPLC instrument

Boulder, CO

• Functional QC - Solution Affinity assay testing the binding Kd of oligos to protein using radioisotope labelling: HIS-Tagged and Zorbax protein binding assays
• Design and test 80-100bp oligos in qPCR for optimal pairing and formation of primer-dimer
• Gel Electrophoresis on PCR products and for biotin shift assays
• Organizing and keeping inventory of multiple crucial reagents for other research departments.
• Quantification of protein concentrations using nano-orange.

Boulder, CO

• Semi-Automated protein binding assay utilizing the TECAN robotic instrument
• Use of a spectrophotometer to quantify and correctly adjust oligo concentrations
• GDP and GLP laboratory environment

Centennial, CO

• Automated and Semi-Automated testing instruments.o Immucor – NEO for CMV, TPHA, ABO, and RBC pooled cells testso Novartis Diagnostics – Procleix TIGRIS for HIV, HBV, HCV, and WNV testso Bio-Rad – D10 for Hemoglobin A1c.
• Manual testingo Viral Slides and Viral EIAs used to detect a multitude of sexually transmitted diseases in donors.o Manual ABO/Rh typing and Hemoglobin S screeningo ASI RPR cards used to detect Syphilis
• Involved in writing new protocols and regulations per FDA and CAP standards.

• Facilitating collaborative learning and designed study skills for students enrolled in historically challenging courses.
• Developed training sessions for other Tutors and Supplemental Instruction Facilitators.
• Weekly scheduled tutoring for students in all classes of Chemistry.
• Creating mock exams and quizzes tailored to student needs.

• Conducted research involving protein binding affinity of different Synaptotagmin isoforms to a lipid bilayer.
• Specific research involved identifying the diffusion coefficient of Synaptotagmin 7 laterally diffusing across a DOPC/DOPS lipid bilayer.
• Designed and performed cell cultures, PCR, gel electrophoresis, purification and identification of proteins from E. coli.
• Create new protocols for TIRF microscope. Performed tests utilizing a stopped-flow and spectrophotometer.
• Presented findings at the annual Undergraduate Research Opportunity Program (UROP) symposium and local ACS convention

• Laboratory instructor for the following teaching labs:Inorganic Chemistry (Spring 2009, Spring 2010)Physical Chemistry I (Spring 2010)Physical Chemistry II (Fall 2011)Honors General Chemistry I (Fall 2011, Fall.
• Performed cell cultures, PCR, protein and DNA gel electrophoresis, TIRF microscopy and FRET experiments for students and material analysis using MS, IR, HPLC, FPLC, FT-IR, and Spectrophotometer machines

Master Of Science (M.S.), Chemistry

Thesis Title: Single-Molecule Analysis of Synaptotagmin 7 Lateral Diffusion across a Supported Lipid Bilayer. Research focused on the.

Bachelor Of Science (B.S.), Chemistry

Activities and Societies: Golden Key, American Chemical Society