Karolos Douvlataniotis Email & Phone Number

Oslo, Norway

Our group focuses on the function of DNA methylation in the biology of human CD4 T-cells and it's involvement in T-cell malignancies.

Linkoping, Sweden

"A CRISPR-Cas9 engineered isoform specific TET1 knock-out cell line for investigating the role of TET1 in T-cell biology"Using a novel fluorescence-assisted CRISPR-Cas9 approach, that was developed during my 1st year project, we generated a TET1, isorform specific KO Jurkat cell line, in order use it as a model system to dissect the function of TET(s) in T-cell differentiation and T-cell malignancies. Lab work involved:-Designing of gRNAs-Transfections via electroporation-FACs sorting-Mutation detection assays (T7 endonuclease based)-Dot & Western Blots-Bacterial cloning of mutated regions and sequencing-qPCR to study gene expression

Linkoping, Sweden

Student Ambassador for LiU, involved with promoting the university to International Students and helping out new or prospective students. Tasks involve:- Blogging- Assisting the International office in phone campaigns / webinars / information to prospective students-Alumni recruitment

Linkoping, Sweden

Project work as part of my Master's degree, extended by a 3 month scholarship (Stiftelsen för barnmedicinsk forskning stipend).Title: "Dissecting the role of Thymine DNA glycolysase (TDG) in epigenetic reprogramming of human CD4+ T-cells". Supervised by Colm Nestor The project's aims involved the following techniques:- Developing & optimizing an ELISA-based approach for the detection of DNA modifications (mC/hmC/fC/caC)-Establishing and optimizing a protocol for a novel CRISPR-Cas9 approach-Cell culturing (HEK293T/Jurkat)-Knockdown/outs using siRNAs, TALEN or CRISPR-Cas9 approaches, via chemical transfections or electroporation -Western/Dot Blots, Gene expression analysis by qPCR, Flow cytometry, agarose gels- Enzymatic mutation detection assys / sequencing- FACs sorting (Aria III)- Bacteria transformation & plasmid cloning

Larissa, Greece

Routine clinical work, with lotations between the labs of Microbiology, Haematology,Biochemistry, Blood Transfusion & Immunology.

Bragança, Portugal

Project title: “Identification and characterization of Honey bee (Apis millifera) pathogenic fungi and bacteria – Erasmus Placement"

Master'S Degree "Experimental And Medical Biosciences", Medical Biology

Bachelor Of Science (Bsc) - Medical Laboratory Technology, Biomedical Sciences