Keith Haskell Email & Phone Number

St. Louis, Mo, Us

St. Louis, Mo, Us

St Louis, Missouri, United States

St. Louis, Mo

• Generate genome edited cell lines using CRISPR-Cas9 and viral transduction. • Experienced in the evaluation of cell lines using molecular biology techniques such as PCR and qRT-PCR Taqman analysis. In addition to using the analysis tools, IGV and OutKnocker to evaluate NGS data. • Support flow cytometry needs for cell line development. Proficient in using FlowJo software for the analysis of flow data and its presentation to colleagues. • Provide customer updates and summary reports for cell lines generated.

Groton, Ct

• Responsible for the generation of genome edited human ESC and iPS cell lines using CRISPR-Cas9, Zinc Finger Nuclease, and TALEN technologies. • Developed an improved process flow to incorporate cell sorting into cell line development resulting in a significant reduction in the time in takes to generate a line. • Performed flow cytometry and qRT-PCR Taqman analysis to ensure the quality of the stem cell line generated.• Supported flow cytometry needs for any cell line development. Proficient in using FlowJo software for the analysis of flow data and its presentation to colleagues.

Groton, Ct

• Screened RNAi libraries in differentiating human mesenchymal stem cells (hMSC) to identify potentially novel drug targets for osteoporosis, obesity, and cancer. • Developed a robust method for the expansion of human adipose stem cells (hASC) for the creation of working stocks and transferred technology to reagent provision group for distribution. • Partnered with research units for the screening of hMSCs and hASCs to identify optimal donor lots for in vitro models. • Developed a protocol to differentiate mast cells from human CD34+ bone marrow stem cells as a drug safety in vitro model. QC of lots by measuring histamine release by ELISA and detection of surface markers by flow cytometry. Screened compounds in this model to address drug safety.• Scaled up the production of pancreatic endocrine progenitors derived from hESCs in 6 well plates to spinner flasks. • Devised a mouse ES derived adipocyte differentiation protocol to be used as a model to predict the in vivo model prior to delivery. • Generated genetically modified mouse ESC lines along with contributions and improvements to the lab’s SOPs.

Groton, Ct

• Developed and improved processes for the generation of viral and parasitic antigens for veterinary vaccine formulations in a GLP and GMP setting. • Generated large scale cell culture systems such as spinner flasks, roller bottles, cell factories, and stirred bioreactors (10L, 50L, and 100L). • Managed the setup, monitoring, and cleaning up of bioreactors between batches. • Regular communication with Discovery and Manufacturing for effective process transfer.

St. Louis, Mo

• Developed and optimized serum-free (SF) and protein-free (PF) media for cell lines used in vaccine and protein production. • Generated optimal media formulations and culture conditions for the following cell lines; MDCK, MDBK, chick embryo fibroblast, Wi38 fibroblast, hybridomas, and CHO. • Tested media formulations in hollow fiber and stirred bioreactors. • Provided support for customers, technical services, QC, QA, and production.

St. Louis, Mo

• Designed and executed scaling-up chemical processes from the fume hood to 1000 gal reactors. • Routinely performed various methods of chromatography and purification techniques. • Performed and interpreted analytical evaluation of products and intermediates.

Ma, Biology

Bs, Chemistry