Keith Yu Email & Phone Number

Redwood City, Ca, Us

Redwood City, California, United States

Executed on process development of ctDNA cancer diagnostic products. Supported clinical labs via troubleshooting and continuous improvement of NGS-based workflows. Collaborated with cross-functional teams and managed teammates and resources to research, develop, and implement process improvements.● Utilized NGS data metrics to identify and resolve sequencing performance issues● Adapted NCRs and JIRA tickets into long-standing workflow optimizations● Created PCR-based contamination analysis procedure to aid in troubleshooting● Developed modular performance qualification methods for Hamilton STAR liquidhandlers, saving tens of thousands of dollars per PQ event● Created NGS QC analysis templates via MS Excel to easily report out data● Codified process improvements into SOPs within a clinical framework

San Francisco Bay Area

Developed high throughput cellular functional assays and screened small molecules for various projects in the interest of advancing cancer therapies. Example assays include cell proliferation, protein expression, cell signaling, enzyme activity, and siRNA transfection mechanism of action. Authored a number of SOPs and trained peers to improve lab function.● Implemented automation (Tecan D300e, Beckman Echo, BlueCatBio BlueWasher) to improve assay throughput and consistency, saving weeks of time and thousands of dollars per failed experiment● As the lab Safety Officer, implemented internal lab inspections to reduce EH&S inspection findings from an average of 5 to < 1● Built up the research lab from scratch, after Discovery Biology was previously shuttered, including sourcing equipment and reagents, as well as building out infrastructure in support of streamlined lab function● Participated in numerous sub-teams and committees, including:○ Entertainment ○ EXEL-Connect ○ Safety Evacuation ○ Diversity

San Francisco Bay Area

San Francisco Bay Area

Worked with CHO cells to produce, purify, and characterize bispecific antibodies against various disease targets through the generation of stable cell lines, including plasmid expression in E. coli, transfection, single cell cloning, flow cytometry, and HPLC for antibody purification. Maintained host cell lines in suspension for distribution and managed lab supplies and instruments, including repair and maintenance.● Applied 5S Lean methodology to a variety of tasks and initiatives to improve process flow and reduce costs● Provided solutions to unexpected logistical situations in the lab, minimizing lost time and maximizing the group’s productivity● Drove lab safety improvements by performing frequent lab inspections across multiple lab spaces, presenting findings to the Safety committee● Supported the achievement of company-wide sustainability goals by implementing additional recycling streams for nitrile gloves, disposable lab coats, paper waste, etc● Significantly reduced cross-campus materials spend by working with Business Operations to come up with savings strategies● Reduced capital equipment maintenance and saved space by assisting in equipment reduction initiatives

Berkeley, Ca

Maintained and utilized cytometers in high throughput cell sorting, antibody drug screening, and cell line development assays. Analyzed multicolor FACS data via BD Diva, FlowJo, Graphpad Prism, and MS Excel software. Trained additional cytometer users as necessary to improve throughput.● Created quick-reference guide for CANTO II to decrease troubleshooting time from hours to minutes● Created quick-reference guide for common laboratory supplies to reduce time spent on purchasing● Developed protocol for viability staining in common binding assays to improve confidence in data output● Trained additional cytometer users as necessary to improve throughput

Greater San Diego Area

Assisted graduate students with research on tissue engineering, including cell culture, iPSC culture and differentiation, chemical synthesis for scaffold fabrication, stereolithography, Piranha cleaning, and lab supply management. Additionally trained new students on basic lab techniques● Rewrote outdated protocols, resulting in smoother lab function.