Kendall Walker Phd. Email & Phone Number

London, England, GB

Le Genest-Saint-Isle, FR

Le Genest-Saint-Isle, FR

Paramus, New Jersey, US

Responsible for managing multiple concurrent pre-clinical research projects for biotech, pharma, & non-profit organizations focused on Huntington’s Disease, Parkinson’s Disease, Muscular Dystrophy & Spinal Muscular Atrophy.Direct all aspects of client projects from advising on experimental design, preparing study proposals & pricing, resource allocation.

Paramus, New Jersey, US

Paramus, New Jersey, US

Boston, MA, US

• Laboratory specializes in the identification of BACE1 regulatory mechanisms for the treatment of Alzheimer’s Disease (AD). Advisor: A/Prof. Giuseppina Tesco I am currently investigating the pathological role of BACE1.
• Demonstrated a crucial role for the BACE1 regulating molecule (GGA3) in the aging brain
• Identified & published the first known molecular mechanism (caspase mediated depletion of GGA3 & GGA1) responsible for regulating BACE1 activity & Aβ production following TBI.
• Demonstrated that GGA3 haploinsufficiency (as occurs in the brains of AD patients) is an important risk factor for the development of chronic neurodegeneration following TBI.
• Developed an improved fluorimetric method for measuring BACE1 activity in mouse brain tissue, a notoriously difficult assay to perform accurately. Published J. Neurosci. 2012.
• Identified a novel behavioral phenotype associated with GGA3 induced elevation of BACE1 in the GGA3KO mouse model (manuscript in preparation)

Boston, MA, US

• Molecular Neurodegeneration Laboratory, Genetics and Aging Unit.Lab specializes in the identification of BACE1 regulatory mechanisms for therapeutic treatment of Alzheimer’s DiseaseAdvisor: A/Prof. Giuseppina.
• Research demonstrated an important molecular mechanism for BACE1 elevation and neurotoxic Aβ production (caspase-mediated depletion of the intracellular trafficking protein GGA3) and provides a novel therapeutic target.
• Collaborated with Drs. Michael Whalen and Rebekah Mannix of the Centre for Neuroscience at MGH to examine the effect of APOε4 on outcome following TBI. Performed Aβ ELISA’s that demonstrated that the APOε4 gene.

Boston, MA, US

• Cecil B Day Neuromuscular Lab, Massachusetts General Hospital.Lab focuses on the genetics of neuromuscular and neurological disorders, in particular ALS. Advisor: Prof. Robert H Brown JrAngel Fund Fellow 2007 - awarded.
• Performed in vitro studies to silence mutant G93A SOD1 using siRNAs electrostatically coupled to a novel cell penetrating peptide based upon the rabies virus glycoprotein (RVG).
• Designed and expressed recombinant neurotrophic factors IGF-1 and VEGF with the RVG sequence as a protein transduction domain using the Baculovirus expression system (BEVS).
• Supervised a graduate student from Harvard College for the practical component of her degree.Techniques used include: plasmid design and construction, recombinant protein production using BEVS, gene silencing using.

Perth, Western Australia, AU

• Molecular Neurogenetics Laboratory, Western Australian Institute for Medical Research (WAIMR) specializes in the genetics and biochemistry of rare neuromuscular diseases.Lab Head: Prof. Nigel G Laing
• Identified disease-causing mutations for nemaline myopathy and congenital fibre type disproportion. [Published Ann. Neurol. 2004; Ann. Neurol. 2007]
• Collaborated with the Western Australian Health Department to optimize their southern blot assay for myotonic dystrophy (DM1) in clinical samples.
• Produced and purified recombinant thin filament proteins (skeletal α-actin, α-tropomyosin-1 and α-tropomyosin-3) using the BEVS for functional studies of congenital myopathy pathogenesis. [Published Biochem. Biophys..
• Initiated and completed a candidate gene-screening project of an Australasian cohort of non-SOD1 familial and sporadic ALS patients. This study was awarded a 25K grant-in-aid from the Motor Neuron Disease Research.
• Responsible for training research assistants and graduate students. Co-edited manuscripts for Sciencedit, a company specializing in editing and writing assistance for researchers with english as a second.

Perth, Western Australia, AU

• This research Institute focuses on uncovering the genetic and environmental causes of disease. Advisors: Prof. Nigel G Laing & Prof. John Howell
• Characterized the molecular and biochemical phenotype of the ovine model of McArdle’s disease, a metabolic disease affecting skeletal muscle. Analyzed the ability of butyric acid to re-express the fetal isoform of.
• Demonstrated that exploitation of gene redundancy through the re-expression of the fetal isoform of glycogen phosphorylase via myotoxin induced muscle regeneration is a potential therapeutic strategy.
• Demonstrated that injection of AAV’s expressing muscle glycogen phosphorylase into the muscle of McArdles’ affected sheep was able to restore glycogen degradation and the expression of functional glycogen phosphorylase.
• Trained research assistant/junior graduates. Designed/implemented vivisection (animal ethics) registrations.Techniques used include: recombinant protein production (BEVS), Purification of recomb. and non recomb..

Sub-contractor of custom research services for Molecular Research Technologies (Pty Ltd).

Bsc (Hons), Biotechnology

Phd, Veterinary Biology And Biomedical Science