Kenny Shimada Email & Phone Number

San Diego, California, United States

San Diego, California, United States

• Key member in the cell line development team to generate stable HEK293T Cell lines capable of producing Adeno-associated Virus (AAV)• Produced and cultured cell lines via CRISPR/Cas9 homology-independent targeted integration that achieved target level of productivity• Generated monoclonal colonies from cell pools via Cellraft• Sole operator screening 5000+ samples for quantification of viral genome of AAV via dPCR andddPCR with a TAT of 48-72 hours for 200-300 samples• Trained other research associates on dPCR and ddPCR protocols for quantification of viral genome• Created analytics template and protocol for sample submission and reporting system used by the broaderteam• Performed dPCR genotyping and flow cytometry to exam stable pool gene copy number and expressionlevel• Participated in developing multiple assays to assist research discovery

Grossing Laboratory Technician• Provided gross examination of various tissue specimens (i.e. gastrointestinal tract, gynecological, vasectomies)• Operated and changed reagents of tissue processors• Performed tissue embedding of processed samples in paraffin wax• Tabulated logs and records in accordance to CAP accredited standards COVID-19 Laboratory Technician• Diagnosed nasal swab test kits for SARS-CoV-2• Operated Tecan Liquid Handlers for high-throughput aliquot• Extracted RNA/DNA using KingFisher Flex Purification System• Conducted high-throughput RT-PCR using QuantStudio 12K Flex Real-time PCR System• Assisted R&D Department in Loyola COVID Study to expedite analysis of PCR results

Irvine, California, United States

• Inputted patient data into XIFIN LIS and created technical sheets for pathologists/clients

San Diego, California, United States

• Managed student workers with different tasks throughout their shift• Provided customer service over the phone and assisted students with inquiries at the front desk