Assistant Research Specialist
Clemson University
Investigated the kinetic properties and affinities of SMUG1, a DNA glycosylase repair enzyme, in restoring the functionality of deaminated base structures. This was accomplished through a methodic protocol using varying forms of SMUG1, created by using a step-wise mutation at different amino acid sites surrounding the enzymes active site.Experience in wide breadth of laboratory techniques: Polymerase chain reaction and cloning of PCR products, electroporation, gel electrophoresis (polyacrylamide and agarose gel beds - vertical and horizontal), gene knockout procedure, restriction enzyme digestion and ligation, column purification, standard titration and dilution technique, DNA sequencing, plasmid mapping, mass spectrometry, and plant tissue hybridization.Journal Publishing:“Insights from xanthine and uracil DNA glycosylase activities of bacterial and human SMUG1: switching SMUG1 to UDG, J Mol Biol, 385: 761-778.”