- Prepared, cultured, and validated CRISPR cell lines expressing GPCR membrane proteins for use in flow cytometry using the BD Accuri CSampler Plus and the Wolf Cell Sorter (Nanocellect).- Led two GPCR antibody discovery projects utilizing both phage display and in vivo methods including experimental design,monitoring project timelines and data analysis.- Screened for GPCR target-specific antibodies via IgG variable region cloning, high throughput ELISA and PBMC isolation from immunized… Show more - Prepared, cultured, and validated CRISPR cell lines expressing GPCR membrane proteins for use in flow cytometry using the BD Accuri CSampler Plus and the Wolf Cell Sorter (Nanocellect).- Led two GPCR antibody discovery projects utilizing both phage display and in vivo methods including experimental design,monitoring project timelines and data analysis.- Screened for GPCR target-specific antibodies via IgG variable region cloning, high throughput ELISA and PBMC isolation from immunized rabbits.- Generated 10 specialized phage display libraries for GPCR antibody discovery and screened 400+ clones against in-house CRISPR cell lines using high throughput flow analysis (BD Accuri), phage ELISA and phage Western blot.- Established protocols for phage display process including library generation, scFv purification, and target-specific screening.- Instrumental in establishing a working laboratory for a startup company setting including acquiring laboratory equipment, creating accounts and relationships with vendors, outfitting benches, planning space use and up to and including delivering equipment from warehouses to our lab.- Actively served on scientific presentation committee aimed at improving employee presentation skills for professional development. Organized ‘lunch and learn’ seminars to further scientific discussion among peers. Show less

•Collaborated with 6-member team to create a proprietary peptide-based Western blot for validating post-translationally modified targets (Patent filed August 2020, UK)•Isolated B cells from immunized rabbits and extracted mRNA to find clones targeting COVID-19 SPIKE protein and its variants•Validated antibodies in phage-display pipeline via high throughput western blotting with up to 100 blots per session•Performed high throughput 96-well and 384-well ELISAs in B-Cell cloning… Show more •Collaborated with 6-member team to create a proprietary peptide-based Western blot for validating post-translationally modified targets (Patent filed August 2020, UK)•Isolated B cells from immunized rabbits and extracted mRNA to find clones targeting COVID-19 SPIKE protein and its variants•Validated antibodies in phage-display pipeline via high throughput western blotting with up to 100 blots per session•Performed high throughput 96-well and 384-well ELISAs in B-Cell cloning pipeline•Maintained bacterial cultures and incorporated PCR, RT-PCR, Infusion/Transformation, extraction and purification techniques to clone and isolate DNA•Worked alongside research team to develop method for finding functional antibodies against GPCRs on an NIH grant-funded project•Performed a variety of plate-based kit assays to test the functionality of phage-display derived pipeline antibodies for NIH grant-funded project•Performed FPLC and assessed antibody yield using the ÄKTA pure purification system and Octet Red96 system for over 40 antibodies•Completed standard and high throughput 293fectin-based transfections for 100+ samples•Treated and prepared mammalian cell lysates for in-house post-translational modification antibody validation•Wrote multiple laboratory protocols and trained employees within and across teams on equipment usage and standard operating procedures•Actively served on Quality Assurance committee editing SOPs, and Employee Satisfaction committee to improve employee morale•Contributed to the development of the Electronic Lab Notebook/LIMS system on site to improve lab workflow across sitesEquipment : Octet Red96, ÄKTA pure purification system, LI-COR Odyssey/CLx and ChemiDoc MP Imaging systems, Janus G3 Varispan + MDT Automated Workstation, Dropsense 96, Countess Automated Cell Counter, Biotek Microplate 405 LS Washer, Trans-Blot Turbo System, Criterion Dodeca Cell Show less

Thesis Title: Early life maternal separation induces anxious behavior and changes in inflammatory signaling and actin-related protein expression in rats•Coordinated and executed maternal separation project for inducing anxiety/depression in rodents. Directly monitored the maintenance and caring for rodent subjects across several cohorts•Investigated the expression of proteins linked to depression/anxiety signaling pathways via Western Blot analysis•Directly involved in sacrifice… Show more Thesis Title: Early life maternal separation induces anxious behavior and changes in inflammatory signaling and actin-related protein expression in rats•Coordinated and executed maternal separation project for inducing anxiety/depression in rodents. Directly monitored the maintenance and caring for rodent subjects across several cohorts•Investigated the expression of proteins linked to depression/anxiety signaling pathways via Western Blot analysis•Directly involved in sacrifice, perfusion, fixation and sectioning of rodent brains for histological analysis•Utilized fluorescent immunohistochemical staining and confocal microscopy to observe neuron-glia interaction•Interpreted immunohistochemical and behavioral data through qualitative and statistical analysis. Incorporated MATLAB coding into neuronal/glial analysis•Trained new and existing lab-mates in animal care and laboratory techniques, including confocal microscopy, Western Blot analysis, fluorescent immunostaining, tissue fixation and histology•Enhanced existing laboratory protocols, designed new experiments, and delegated tasks to lab members. Maintained antibody and reagent quality control through testing and product research•Thorough library research and literature review regarding inflammatory pathways, neural circuitry, mood disorders, and synaptic processing. Attended weekly laboratory meetings and presented articles in journal club discussions Show less

•Implemented therapeutic exercises to improve range of motion in post-surgery and injured patients •Performed ultrasounds and electrotherapy as pain relief techniques and worked alongside medical personnel to explore patient treatment options•Measured patients’ respiratory and circulatory efficiency, including blood pressure and heart rate, prior to therapist evaluation. •Administrative duties included managing patient referrals and records, documenting interdepartmental meetings… Show more •Implemented therapeutic exercises to improve range of motion in post-surgery and injured patients •Performed ultrasounds and electrotherapy as pain relief techniques and worked alongside medical personnel to explore patient treatment options•Measured patients’ respiratory and circulatory efficiency, including blood pressure and heart rate, prior to therapist evaluation. •Administrative duties included managing patient referrals and records, documenting interdepartmental meetings, scheduling appointments and greeting patients Show less

•Organized and supervised stimulating activities promoting teamwork and social skills children ranging from ages 5- 16•Directly managed support staff daily schedules and implemented the use of raw and locally sourced materials to decrease overall program budget by 30%.•Fostered teamwork and social interaction through outdoor recreation, arts and crafts and culinary arts