Kia Vest Petersen Email & Phone Number

Birkerød, Dk

Denmark

The GMP facility where I am working is closing down and focus since end of May has therefor been:- PM QMS track - decommissioning of GMP facility- Closure of EM and PW monitoring including documentation of this- Define requirements for facility towards decommissioning and coordination of implementation

Hørsholm, Capital Region, Denmark

- Environmental and purified water monitoring lead – Highlighted achievements: - PQ followed by implementation of routine monitoring - Alignment of EM program to new version of Annex 1 - Ongoing coordinator of sampling and analysis at external laboratory - Ongoing trending and reporting- Cleanroom lead – Highlighted achievements: - Defined requirements for periodic classification testing and coordination/review of testing - Handling of changes to cleanroom status - Defined requirements for facility cleaning including risk assessment for validation of disinfection Agents- Define requirements for the cleanrooms in connection with line clearance / product changeover- Author and review of GMP documents like policies, SOPs, protocols and risk assessments- CRs, Deviations, CAPAs and documentation- Training coordinator

Copenhagen Area, Capital Region, Denmark

- PM and implementor for design, setup and implementation of an ELN system and an DMS for both GMP and non-GMP documents- Author and reviewer of policies and SOPs- Microbial knowledge support for setup of research and GMP cell bank procedures

Hørsholm

Assisting in building up Bacthera's cGMP quality system in a 1 month Internship

Denmark And Iceland

AlcoWhey was founded in November 2017 with the ambition to turn the vast amounts of whey residuals into alcohol by Lactic Acid Bacteria fermentation. I was employed in the company's start-up period to work with development and quality:▪️ Development of ethanol producing Lactococcus lactis strains▪️ Set-up of fermentation conditions and upscaling▪️ Risk assessment, compliance and QC set-up for produced ethanolMetabolic engineering | Molecular biology | Adaptive evolution | Mutagenesis | Screening | Optimization of fermentation media (laboratory scale) | Upscaling of fermentation to 500 L | HPLC | Identification of relevant purity and regulatory requirements | Method development for identification of volatile compounds (with external partner at KU) | Retrieval of offers from external analytic laboratories

Hørsholm

I worked with ensuring the quality and compliance of raw materials to be used in the production of food and pharmaceutical products. My main task was risk assessment and approval of new raw materials in accordance with ISO 22000 and GMP:▪️ Safety (chemical, microbiological and HACCP/GMP)▪️ Regulatory requirements▪️ Technical requirements▪️ Business requirements▪️ Drafting of raw materials specificationsMy other tasks included:▪️ Optimization of work tools and standard operating procedures▪️ Support function for internal stakeholders▪️ Handling of change control and deviations

Hørsholm

I compared gene expression in Bacillus subtilis strains using qPCRGrowth experiments | RNA purification | cDNA generation | Test of primers | qPCR and analysisTime-limited internship

Ballerup

Based on a focus on Sustainable Biotechnology and biorefineries I worked with:▪️ Identification of environmental filamentous fungi isolates▪️ Screening of filamentous fungi for fungal strains producing enzymes or acids of interest Cultivation of fungi | Extraction and purification of fungal genomic DNA | ITS PCR | Sequence data analysis | Screening | Enzyme assays

Denmark

I worked with enzyme discovery, where I was searching for novel acid stable serine peptidases. The work included both protein chemistry and molecular biology:▪️ Screening for novel enzymes by purification of enzymes from wild type bacteria cultures▪️ Screening for novel enzymes by molecular screening using PCR ▪️ Heterologous gene expression in Bacillus subtilis ▪️ Purification of enzymes▪️ Characterization of enzymes (pH and temperature profiles)Cultivation of bacteria | Isolation of DNA | Primer design | PCR | Gene walking | Cloning | DNA sequences data analysis | Gene expression in Bacillus subtilis | Purification of enzymes | Enzyme assays All work was done at Novezymes in the two R&D departments Bacterial Screening and Protein Biochemistry

I worked in the R&D department Protein Biochemistry in the laboratory doing:▪️ Enzyme activity assays▪️ Preparation of culture fluids for purification of proteins▪️ Purification of enzymes▪️ Preparation of buffers

Gmp

GMP education at Pharmakon (Danish College of Pharmacy Practice)

Ph.D., Biotechnology | Microbiology | Metabolic Engineering | Molecular Biology

I investigate the potential of Lactococcus lactis as a platform organism for production of biofuels and-chemicals with a focus.

Master Of Science In Engineering, Biotechnology, Master Thesis: 12

Activities and Societies: Crew, PF’s student house S-huset | Crew chairman, PF’s student house S-huset | Member of PF's committee.

Mathematical Line

Subjects at advanced level: Mathematics, physics and chemistry