Kieran James Sharp Email & Phone Number

Westminster, Co

I design and implement Wordpress-based websites and blogs. I also develop marketing graphics and materials for print and web campaigns and create custom social media action plans and perform social media and blogging related tasks for clients.

Greater Denver Area

As a mentor for MHCD's Voz y Corazon Youth Suicide Prevention Program, I facilitate art-based non-therapy support groups for at-risk youth. Responsibilities include youth engagement and recruitment, scheduling, collecting appropriate permissions for participation, record keeping, data collection as requested by MHCD, attending mentor trainings and meetings, conforming to program standards and requirements, coordinating youth transportation via MHCD's contracted taxi program as needed, attending annual Suicide Prevention Gatekeeper training, and coordinating as needed with host organizations that provide space and time for groups to meet.

Tissue culture and cell maintenance of adult mesenchymal stem cells for autologous clinical use, processing of human blood products and bone marrow, sample preparation for clinical use, cryopreservation, cell counting, thorough documentation and record keeping, writing SOPs, assist in training new lab employees, working without supervision, adhering to GLP and GMP standards, following HIPPA regulations, general laboratory maintenance.

Denver, Co

Research Focus: investigate insulin signaling in RASM cells in relationship to atherosclerosis in Type II Diabetes.Lab Techniques and Equipment Used: micropipetor and autopipetor use, serial dilutions, microscopy, PCR, restriction digests, SDS-PAGE (gel electrophoresis), Western Blotting, spectrophotometry, luminometry, siRNA (RNAi), tissue culture, bacterial culturing, plasmid purifications/preps, immunoprecipitation (IP) for sample preparations, cell lysis, transfections, transformations, BCA assays, RNA collection, Southern and Northern blotting, immunocytochemistry (ICC), densitometry and typhoon analysis.General Lab Duties: making stock solutions, autoclaving, pouring plates, pouring agarose gels, plasmid preps/purifications.

Boulder, Co

Production Duties: receiving, inspection/acceptance, record keeping/maintenance, rework, packaging, labeling, shippingQuality System Duties: planning and scheduling, purchasing, material identification and traceability, document control, labeling control, non-conforming material control, training, revising and writing procedures.Other Duties: comply with ISO quality system standards, medical device GMP (QSR), Canadian Medical Device Regulations; perform customer service and interact with suppliers, responsible for accurate product knowledge.