Enzymes

Immunotechnology - Screening & Cell TechnologyScreening, assay development and automation- Trained in programming complex liquid handling robots, Hamilton STAR, Nimbus and Biomek 4000- Assay and liquid handling using robots.- Development of new automated methods in 384-well format.- Screening of antibodies/proteins in Haemophilia projects.- Development and testing in chromogenic enzymatic blood coagulation cascade assays- Proteolytic Assays.- AlphaLISA Cytokine… Show more Immunotechnology - Screening & Cell TechnologyScreening, assay development and automation- Trained in programming complex liquid handling robots, Hamilton STAR, Nimbus and Biomek 4000- Assay and liquid handling using robots.- Development of new automated methods in 384-well format.- Screening of antibodies/proteins in Haemophilia projects.- Development and testing in chromogenic enzymatic blood coagulation cascade assays- Proteolytic Assays.- AlphaLISA Cytokine Assays- High Throughput screening of 10.000+ compounds.- Reporter Gene Assay and cell-line handling. Show less

- Molecular biology- qPCR- Small molecules screening - DNA encoded chemistry- AlphaScreen- Cell culture experiments

In ALK Research I have worked with:- Assay development: ELISA on human and murine serum and saliva samples, testing various commercial assay platforms.- Antibody analysis/quantification: ELISA, Biacor, ADVIA Centaur and Mesoscale.- Tecan liquid handling robot- Developing assays for automation handling.- Animal experiments - Lung Function measurements using pulmonary plethysmograph- Antibody purification on magnetic beads- Electrophoresis - SDS-PAGE, IEF- Protein… Show more In ALK Research I have worked with:- Assay development: ELISA on human and murine serum and saliva samples, testing various commercial assay platforms.- Antibody analysis/quantification: ELISA, Biacor, ADVIA Centaur and Mesoscale.- Tecan liquid handling robot- Developing assays for automation handling.- Animal experiments - Lung Function measurements using pulmonary plethysmograph- Antibody purification on magnetic beads- Electrophoresis - SDS-PAGE, IEF- Protein purification - SEC, Äkta- Growing and maintenance of house dust mite cultures- Endotoxin analysis- Mass Spectrometry - Bruker MaXis UHR-Qq-TOF - Business Project Manager in IT-Project, ELN (Electronic Labnotebook) implementation in Denmark and Spain. Show less

PNAD was a molecular diagnostic company focusing on PNA molecules for human clinical diagnostics – Closed in 1999In the Assay Development team I worked with:- PCR , sample preparation and PCR development /optimization- Gel electrophoresis on peptides, DNA and PNA- Photo-immobilization of PNA to solid surfaces- DNA/PNA hybridization measurements (Tm)- Feasibility study on magnetic beads at Boehringer Mannheim in Mannheim

- Rubella diagnostic - IgG/IgM ELISA- HIV diagnostic - ELISA/EIA and Western blotting- Production of Polyacrylamid gradient gels and Western blotting strips for HIV-diagnostic.- T-cell helper/supressor ratio - Flow cytometry- Immunofluorescens microscopy on T-lymphocyte subpopulations.- Growing of primary cell-cultures- Propagation of HIV infected cells in Biosafety-3 laboratory- provide in-house training for different types of national and international laboratory… Show more - Rubella diagnostic - IgG/IgM ELISA- HIV diagnostic - ELISA/EIA and Western blotting- Production of Polyacrylamid gradient gels and Western blotting strips for HIV-diagnostic.- T-cell helper/supressor ratio - Flow cytometry- Immunofluorescens microscopy on T-lymphocyte subpopulations.- Growing of primary cell-cultures- Propagation of HIV infected cells in Biosafety-3 laboratory- provide in-house training for different types of national and international laboratory staff. Show less