Kimberly Reese Email and Phone Number
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Stem cell specialist with 10 years of experience in mammalian cell culture and molecular biology. I am passionate about regenerative medicine and using stem cell technology to enable the clinical use of engineered human cells, tissues and organs.I am currently managing the Cell Production and Process Development team at United Therapeutics’ Organ Manufacturing Group in Manchester NH where we are scaling up and modifying iPSC differentiation protocols to be GMP-compatible. Previously, I worked at the Advanced Regenerative Manufacturing Institute (ARMI) collaborating with member organizations to scale-up, close, and automate tissue/organ engineering protocols for manufacturing. I also worked as a Research Associate at Omega Therapeutics and Fulcrum Therapeutics differentiating stem cells to neural and cardiac lineages to test novel therapeutics for treating rare diseases. I obtained my Master’s in Biology at Plymouth State University studying population genetics and the fibrosis-related effects of single nucleotide polymorphisms in the Connective Tissue Growth Factor (CTGF) gene.
United Therapeutics Corporation
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Manager Of Cell Production And Process DevelopmentUnited Therapeutics Corporation Jun 2023 - PresentSilver Spring, Maryland, UsFormerly Lung Biotechnology PBC
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Scientist - Process DevelopmentAdvanced Regenerative Manufacturing Institute (Armi) | Biofabusa Aug 2022 - Jun 2023Manchester, New Hampshire, Us -
Associate Scientist - Process DevelopmentAdvanced Regenerative Manufacturing Institute (Armi) | Biofabusa Aug 2020 - Aug 2022Manchester, New Hampshire, Us- Technical lead for process development in projects to produce stem-cell derived islet cells in collaboration with the JDRF and Millman Lab at Washington University, stem-cell derived lineages in collaboration with other ARMI members, and scale up of PSC culture in 3D. - Trained ARMI employees in culture and differentiation of pluripotent stem cells- Led technology transfer, process gaps analysis, and risk assessments- Ran experimental studies, engineering tests, process qualifications and process validations to support the development of manufacturing processes- Worked cross-functionally with external clients and collaborators in industry and academia - Led a team of 5 to execute process development studies- Generated SOPs, technical reports/summaries, presentations and detailed batch records- Ran cell culture-based experiments using large-scale 2D culture vessels, bioreactors, and closed counterflow centrifugation. - Assessed process changes using ELISA, flow cytometry, and Process Analytical Technologies (PAT)
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Research AssociateOmega Therapeutics Jun 2019 - Aug 2020Cambridge, Massachusetts, Us- Reprogrammed patient primary cell lines to pluripotent stem cells, characterized clones using ICC and SNP microarrays, and created master and working cell banks- Differentiated induced pluripotent stem cell clones to cardiomyocytes, sensory neurons, and cortical neurons- Developed protocols to extract high-quality RNA and protein from brain and liver samples to support in-vivo studies for multiple programs- Qualified an ELISA assay as fit-for-purpose to characterize serum samples from in-vivo studies- Performed ELISA and qPCR assays to characterize candidate therapeutics in-vitro and in-vivo- Maintained detailed electronic lab notebook records of experiments and results -
Research AssociateFulcrum Therapeutics Dec 2017 - Jun 2019Cambridge, Massachusetts, Us- Maintained, expanded, transduced, and banked wild-type and patient-derived iPSC lines- Differentiated, QC'd, and treated wild-type and patient-derived iNeurons for the Fragile X, Freidrich's Ataxia, and Parkinson's Disease programs- Established a Quality Control SOP for Fulcrum iPSC and iNeuron cell lines- Developed an SOP to cryopreserve iNeurons providing batch consistency and flexibility to end users- Drove a go/no-go decision for two potential FXS targets- Developed protocols on automation systems (HighRes, Agilent Bravo, BioMek) to miniaturize iNeuron cultures into 96- and 384-well formats for screening efforts and use in target validation- Validated qPCR assays for cell health assessment, screening, and target validation efforts- Established an SOP for qPCR assay validation- Implemented new bioregistry and inventory programs and developed protocols to facilitate cell line management and tracking- Analyzed data from studies using Python/Excel/Graphpad Prism- Maintained detailed reports of experiments and results in an electronic lab notebookCell Specialist: December 2017 - February 2018 Research Associate I: March 2018 - June 2019 -
Doherty LabPlymouth State University Jan 2015 - Nov 2017Plymouth, Nh, Us- Researched the role of Connective Tissue Growth Factor (CTGF) in healing and fibrosis- Sequenced CTGF in over 200 individuals and identified six novel SNPs- Predicted the phenotypic effects of SNPs in CTGF and other CCN family genes using bioinformatics- Created plasmid vectors with CTGF and inserted SNPs of interest to be tested in cell culture- Investigated the downstream pathways of CTGF in cell culture using qPCR and western blottingNH-INBRE Laboratory Manager/Coordinator: Summer 2017Graduate Researcher: Fall 2015 - Spring 2017NH-INBRE Laboratory Manager/Coordinator: Summer 2016NH-INBRE Summer Research Fellow: Summer 2015Undergraduate Student Researcher: Spring 2015Personal responsibilities include: Troubleshooting, protocol optimization and writing, DNA extraction from buccal cells, PCR, DNA purification, gel electrophoresis, SNP identification with PolyPhred, maintaining ATCC NIH/3T3s, mouse primary and immortalized embryonic fibroblasts, and human primary dermal fibroblasts (ATCC BJ) in tissue culture, total RNA extraction from cells, reverse transcriptase PCR, qPCR, statistical analysis in Excel and R, writing abstracts, creating and presenting posters (Harvard NESS, PSU Student Showcase, NH INBRE) and PowerPoints, maintaining a lab notebook, drafting papers to publish. -
Graduate AssistantPlymouth State University Sep 2015 - Jul 2017Plymouth, Nh, Us- Managed the Doherty lab- Taught molecular and cell culture techniques to undergraduate researchers- Taught a Genetics lab and worked as a teaching assistant for Genetics and Molecular BiologyTeaching Assistant Positions:Fall 2015 - Genetics for NursesFall 2016 - Molecular Biology Teaching AssistantSpring 2017 - Genetics- Helped teach three-hour labs once a week- Hosted help sessions for students- Graded quizzes/exams/labs. Teaching Positions:Spring 2016 - Genetics Labs- Taught three-hour labs once a week- Hosted weekly help sessions for students- Graded quizzes/exams/labsLab topics: meiosis & mitosis, karyotyping, genetic crosses & Punnett squares, pedigree analysis, Hardy-Weinberg equilibrium, mutagenesis of Serratia marcescens using UV light, DNA purification, PCR, gel electrophoresis, genotyping, and basic bioinformatics.
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Anatomy & Physiology I Lab TeacherPlymouth State University Sep 2015 - Dec 2015Plymouth, Nh, Us- Taught two classes each week- Covered topics including basic cell biology, tissue identification, the skeletal system, and the muscular system
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Assistant DirectorSmall Wonders Child Care Dec 2013 - Nov 2017- Devised weekly schedules - Conducted weekly payroll- Organized and maintained files for staff and children- Helped prepare and teach preschool activities and curriculum- Computerized timeclock and record keeping systems
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General Surgery ExternshipLittleton Regional Heathcare Jun 2014 - Dec 2014- Observed surgeries in the Operating Room- Learned how to interpret some radiology and lab results- Learned about patient care• Witnessed the entire surgical process several times, from a patient’s initial clinic visit, through surgery, and in their post-op appointment after(About 220 hours)
Kimberly Reese Skills
Kimberly Reese Education Details
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Plymouth State UniversityBiological Sciences -
Plymouth State UniversityMajor - Biology; Minor - Chemistry
Frequently Asked Questions about Kimberly Reese
What company does Kimberly Reese work for?
Kimberly Reese works for United Therapeutics Corporation
What is Kimberly Reese's role at the current company?
Kimberly Reese's current role is Manager Cell Production and Process Development at Lung Biotechnology.
What is Kimberly Reese's email address?
Kimberly Reese's email address is kj****@****uth.edu
What schools did Kimberly Reese attend?
Kimberly Reese attended Plymouth State University, Plymouth State University.
What are some of Kimberly Reese's interests?
Kimberly Reese has interest in Science And Technology, Environment, Education, Health.
What skills is Kimberly Reese known for?
Kimberly Reese has skills like Gel Electrophoresis, Pcr, Qpcr, Research, Powerpoint, Data Analysis, Tissue Culture, Presentations, Teaching, Statistics, Rt Pcr, Rna Isolation.
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