Saravana Kumar Kailasam Mani Email & Phone Number

Los Angeles, Ca, Us

• Currently investigating the role of loss of Y (LOY) chromosome in bladder and prostate cancer; response to immunotherapy• Identified sex specific regulators driving bladder cancer using single cell analysis of carcinogen BBN treated mice• Identified novel molecules crucial for androgen mediated progression of bladder cancer from Next Generation Sequencing (NGS) screens. • Developed pipelines in R and bash scripts for analysis of bulk RNA Seq, single cell RNA Seq and whole genome shRNA screens using cluster computing

West Lafayette, In, Us

• Identified RNA helicase DDX5 as a host restriction factor for Hepatitis B Virus (HBV) biosynthesis and a negative regulator of Wnt signaling• Analyzed genome wide transcriptional differences in drug sensitive vs drug resistant liver cells using RNA Seq and ATAC Seq to identify probable mechanisms of sorafenib resistance in chronic HBV induced liver cancer• Published 2 reviews and 1 first author manuscript

West Lafayette, In, Us

• Identified a novel mechanism of cancer stem cell development in Hepatitis B Virus induced liver cancer• Validated the mechanism in human tissues & mice models, proposed new therapeutic targets in liver cancer• Developed and carried out thesis work independently with minor supervision, managed multiple projects simultaneously• Published 1 first author and 3 co-author manuscripts• Assisted in grant writing and preparation for university funded programs• Presented findings to Advisory Committee annually and at several poster sessions. • Taught undergraduate researchers proper research techniques and documentation• Operating experience using Roche LightCycler 96, Promega Glomax Luminometer, Beckman Coulter FC500, BD LSRFortessa, ViiA7 Real Time PCR system, Olympus FV10i Confocal microscope

West Lafayette, In, Us

• Courses: Principles of Cell and Tissue Design I; Principles of Cell and Tissue Design II• Explained theoretical concepts of tissue organization and function to veterinary students, assessed student’s understanding of the subject, promoted critical thinking through questioning, clarified doubts on course topics through one-on-one interaction.• Commended as knowledgeable in subject and helpful by over 80% of class every semester in end-of-semester course evaluations.

Madison, Wi, Us

• Evaluated and optimized the performance of new chemistries/kits in isolating DNA from different tissues• Performed benchmarking of miRNA purification kits• Optimized a protocol for direct amplification of DNA by qPCR from mammalian cells• Maintained detailed documentation on every project, prepared application notes and reports for internal use

Kharagpur, West Bengal, In

• Assisted PhD students and learnt cell culture, SDS-PAGE and other techniques necessary to work on the project• Applied the techniques I learnt, designed experiments, collected data.• Presented results every semester to a panel of faculty.

Richmond, Va, Us

• Performed literature review, became familiar with human embryonic stem cell culture, cell counting with hemocytometer, immunofluorescence.• Designed experiments, collected data and presented results to a panel of faculty and students at Virginia Commonwealth University.

• Worked under supervision of PhD candidates and learned basic molecular biology and microbiology techniques like streaking, spreading, bacterial transformation, PCR

Doctor Of Philosophy (Ph.D.), Molecular Signaling And Cancer Biology

Bachelor Of Technology (B.Tech.), Master Of Technology (M.Tech), Biotechnology & Biochemical Engineering