Helle Kristensen Email and Phone Number

2018 May - July: I worked with QC in relation to vaccine production and developed qPCR assays in compliance with Ph.Eur :- Assay validation and test off robustness.- Preparation of SOP's in SAP and reading of legislation.- Experimental planning and hands-on work in GMP and biological safety level 2 laboratory environment- Data analysis and thorough documentation

- Project planning and assay design for genomic analysis of Bacteria using Samplix’s Xdrop™ technology for targeted single molecule sample preparation.- Flow cytometry - Bio-Rad S3e™ Cell Sorter.- CLC Workbench – Alignment and evaluation of NGS data from target enriched samples.

Design of highly sensitive and specific Droplet Digital PCR assays for detection of blood-based biomarkers.

Research Scientist in Exiqon’s diagnostic programs: discovery and validation of MDx Biomarkers for prostate and colorectal cancer. Main tasks:- Design, experimental testing, optimization and evaluation of LNA-enriched qPCR assays for profiling of RNA, miRNA, DNA methylation and SNPs. - Clinical research: Study set-up and large-scale data analysis of retrospective biomarker validation experiments on in vitro and clinical patient samples (tissue and biofluids).- Writing SOPs… Show more Research Scientist in Exiqon’s diagnostic programs: discovery and validation of MDx Biomarkers for prostate and colorectal cancer. Main tasks:- Design, experimental testing, optimization and evaluation of LNA-enriched qPCR assays for profiling of RNA, miRNA, DNA methylation and SNPs. - Clinical research: Study set-up and large-scale data analysis of retrospective biomarker validation experiments on in vitro and clinical patient samples (tissue and biofluids).- Writing SOPs and ensuring thorough data documentation.- Daily supervision of 2 technicians.- Cross functional collaboration with R&D, service, production, marketing and External partners in projects teams, plus meeting the deadlines.- Customer contact through service reports and exhibitor boots at international conferences. Show less

Working with DNA methylation and miRNA based biomarkers for prostate cancer- Finishing PhD tasks and projects described below.- Writing and filing patent applications.

PhD thesis: “Identification of novel DNA methylation and microRNA biomarker candidates for prostate cancer diagnosis and prognosis”.Main tasks: - Project planning, experimental designs, hands-on laboratory work and thorough data analysis.- Discovery and characterization of biomarker candidates (DNA methylation, miRNAs and proteins) in vitro and in clinical patient samples. - Various molecular-, tissue- and cell-based methods.- Handling valuable samples from a… Show more PhD thesis: “Identification of novel DNA methylation and microRNA biomarker candidates for prostate cancer diagnosis and prognosis”.Main tasks: - Project planning, experimental designs, hands-on laboratory work and thorough data analysis.- Discovery and characterization of biomarker candidates (DNA methylation, miRNAs and proteins) in vitro and in clinical patient samples. - Various molecular-, tissue- and cell-based methods.- Handling valuable samples from a biobank.- Multi-centre and –country retrospective validation studies.- Great experience with working in ISO 15189 accredited environments. Show less

Hands-on analyses of the quality of cereal samples.