Proficient in Protein and Peptide LC-MS/MS. Expert in operation and maintenance the Thermo Orbitraps and Agilent TOFs. Primary resource for Hydrogen Deuterium exchange (HDX) mass spectrometry, using a Leap HDX Pal System and Thermo Orbitrap Fusion with ETD. Identify epitopes, paratopes, binding sites, and conformational differences by HDX.Experienced in Intact Mass Analysis with Agilent 6200 serious TOFs and 1290 LCs.Evaluate protein sequence coverage, modifications, and glycosylation from enzyme digestion by LC-MS/MS. Built an automated solution for Intact Mass analysis. My software generates complex scripts for liquid handling on a Tecan Evo 100 and full work lists for acquisition and deconvolution on a Agilent 6200 series TOF from 2D barcoded matrix tubes.My HDX software calculates and matches isotope distributions to m/z peaks based on mass and intensity tolerances. Automatically organizes and graphs results in Excel. Colors 3D protein structures.Wrote software that automatically read Outlook emails for intact mass analysis requests. Used to automatically generate report templates with calculated MWs and potential modifications, instrument work lists, folder hierarchies, and summary printouts.Automated the folder hierarchies to extract data from results files, take screenshots, and generate a word document to properly report experiments to the electronic notebook.Wrote C# software that extracts instrument data and queries Oracle databases to automate workflows throughout the department.Generate custom scripts and work lists from 2D barcoded tubes to fully automate liquid handling and mass spec analysis of QC samples using my custom written C# software.

Investigated molecular imaging software to determine issues with new software builds. Managed errors in the Mantis bug database.Constructed complex scripts that pushed the limits of Eggplant software with embedded AppleScript. My automated stress tests checked for errors and inconsistencies. Created a new Serial Number Generator for the CMI software in Java. Developed a cross-platform Graphical User Interface to generate software serial numbers.Performed a flourochrome study in the laboratory and tested products prior to shipping.Produced a case study between Eggplant and Carestream Molecular Imaging.

Created computational techniques to find new translation initiation sites in S. cerevisiae through analysis of mass spectra, ribosome profiling data, and information theory. Discovered high-confidence locations for translation initiation through development of python scripts, SQL scripts, excel macros, ribosome profiling data, open source software, and the SEQUEST algorithm.Wrote an SQL algorithm to determine translation initiation from ribosome profiling data. Developed a machine learning algorithm for interpretation of ribosome profiles.