Kritika Mukherjee Email & Phone Number

Cambridge, Ma, Us

Cambridge, Massachusetts, Us

Cambridge, Massachusetts, Us

Cambridge, Massachusetts, Us

•Cultured primary hematopoietic stem cells and THP-1 AML cell line•Performed electroporation on THP-1 cells with GFP mRNA used as a positive control for various experiments •Delivered Cas9 RNP complexes into hematopoietic stem cells and THP-1 cells. •Designed primers for sequencing utilizing Benchling tools. •Quantified DNA/RNA using the Qubit fluorometer. •Performed Mycoplasma testing in newly thawed cell lines•Optimized PCR conditions to select specific oligos for amplicons containing CRISPR/Cas9 induced indels. •Performed ICE analysis using sanger sequencing to determine editing in the THP-1 cell line and hematopoetic stem cells. •Used CRISPR Cas9 gene editing technology to knock down a protein in hematopoietic stem cells. •Performed data analysis to estimate editing efficiency using CRISPR Cas9 tools.

• Culturing and passing cancer cells lines (213, THP, MCF10A, U87, WBC)• Practiced cell freezing techniques for long term preservation. • Performed MTT cell based assay.• Performed ELISA to confirm a protein sample• Starve media preparation for cell harvesting

Cultured HEK-blue cells. Working on the construct of ha7-C5HT3-TMD1-3. Performed PCR to amplify the new insert. Transformed the dam-/dcm- with the new construct. Extracted DNA from the newly transformed cells for sequencing.

• Performed a research project at undergrad level on Eisenia Fetida (earthworm) using different pharmacological agents by modulating the proteins. • Samples were given for profiling at IIT Bombay lab and orbitrap was used. • Modulated proteins were analysed with the help of graphs • Tissue growth for each earthworm was examined under the microscope using histology techniques. • Extracted proteins using chloroform-methanol technique.

Pune, Maharashtra, In

Performed protein docking and sequence analysis at Mitcon institute.

Acquired training in routine biochemical, haematological, immunological and clinical pathology investigations. Practiced hands on training on sphere random access biochemistry analyser. Learnt and observed the technique of electrophoresis.

Observed autopsy on human body to learn various tissues and organs.Studied organs involved in immune system.

Practiced immunological techniques like ELISA with HRP conjugated antibody to detect an immobilized antigen.Cultured HELA cells in TPP flask.Achieved chick embryo isolation and dyed the amniotic sac.Executed PCR on a short random sequence and analyzed the results with SDS- Page.Performed gel electrophoresis with the help of geldoc .Accomplished Heamagglutination.

Master'S Degree, Biotechnology

Bachelor'S Degree, Biotechnology