Kshama Anantha Kumar Email & Phone Number

Hamburg, Germany

Frankfurt, Hesse, Germany

• Cardiomyocyte differentiation in 2D and 3D models • Routine maintenance and Quality control of Organoids and iPS cell lines • Bulk production of cardiac organoids for different projects • Lab organization and management

Halle, Saxony-Anhalt, Germany

• Culture and maintenance of different human and rat cancer cell lines • Transfection of cancer cell lines for expressing fluorescent proteins • Selection of stable cells using flow cytometry and microscopy • Lab organization and management

Dresden, Saxony, Germany

Project title: Characterization of rs8736 C>T variation on the regulation of MBOAT7 in Non-alcoholic fatty liver disease• Cultured and maintained liver carcinoma cell line ( HUH7)• Transfected them with constructs containing different variants corresponding to MBOAT7 mutation.• Used Dual-luciferase assay to correlate the MBOAT7 gene expression in the cells.• Analyzed MBOAT7 gene expression using qPCR and Western Blot.

Dresden, Saxony

Project title: 2D neural differentiation on anchor polymers treated surfaces• Culturing iPSCs and differentiating them along the neural lineage into early neuroprogenitors• Testing on several peptide combination coatings for optimal differentiation.• Analysis by immunostaining and confocal imaging.

Dresden, Saxony

Project Title: Analysis of sequestered growth factors from GAG-PEG hydrogels• Preparation of GAG-PEG hydrogels• Sequestration and embedding of h-VEGF into the hydrogels• Harvesting and analysis of VEGF from hydrogels using ELISA

Bengaluru, Karnataka, India

Project Title: Developing effective non-viral gene delivery vectors for nucleic acid delivery applications in various mammalian cell lines• Preparation of liposomes by thin-film hydration method• Transfection of Hek and HeLa cell lines with pEGFP using the prepared liposomes as carriers• Determination of transfection efficiency using Fluorescence-activated cell sorting (FACS) ( under supervision)

Bengaluru, Karnataka, India

Project Title: Comparison of immunological and molecular diagnosis of Tuberculosis infection of CNS• Conducted DNA isolation from CSF samples of TB infected patients using CTAB-NaCl method• Amplified this isolated DNA by Polymerase chain Reaction using IS6110 and MPT64 primers• Conducted ELISA for the same samples using Anti-MTSE antibodies.• Comparison of the results and analyzing if individual and/or combined methods are better for accurate results.

Tempe, Arizona

Project Title: Generation of stably transfected H1975 lung cancer cells• Cultured and maintained H1975 lung cancer cells.• Transfected these cells with beta-galactosidase.• Used staining techniques like X-gal and S-gal staining to quantify the expression of beta galactosidase.• Continued study on culturing multicellular spheroids and their staining.

Bengaluru, Karnataka, India

training on molecular biology techniques

Master Of Science - Ms, Molecular Bioengineering

Bachelor Of Engineering (B.E.), Biotechnology