Kurt Franzen Email & Phone Number

Waltham, MA, US

Waltham, MA, US

• Worked independently and collaboratively on analytical development for a cell/tissue therapy project
• Collaborated with teams as a scientific advisor, mentor and supervisor
• Worked in accordance with GMP, wrote SOPs, protocols, reports, and conducted assay qualification and validation
• Utilized: Rheometry, Instron tensile strength, durometry, Spectroscopy, fluorescence, RT, qPCR, ELISA, Lateral Flow, SDS-PAGE, tissue culture, cell culture, NHS, Biuret, BCA, and RNA/DNA purification

Waltham, MA, US

Waltham, MA, US

• Worked collaboratively on projects to improve and develop custom, protein microarray assays.
• Utilized: Luminescence, ELISA, 2470 chip arrayer, Cirascan imager

Chapel Hill, NC, US

• Supervised and worked collaboratively on projects to improve expression and purification of AD, AAV including the manufacture of custom gene therapies
• Utilized: FPLC, qPCR, cloning, CsCl/Iodixanol gradient, tissue culture, transfection, and cGMP

Sant Cugat Del Vallès, Barcelona, ES

• Performed validation study for in-house qPCR, qRT-PCR assays for trace pathogen detection
• Utilized: cGMP, qPCR, qRT-PCR, sterile technique, RNA and DNA purification

• Organized studies involving intellectual property to develop new microarray based diagnostics for chemotherapy selection and clinical study companion diagnostic applications
• Coordinated work at three sites, studied and summarized new technologies and new IP
• Conducted meetings, presented summaries to management, scientific founders and steering committee
• Edited SOP’s and helped initiate the Quality System

US

• Designed, developed, optimized and validated multiple SNP-genotyping qPCR assays for Cytochrome P450 2D6, 2C19, 2C9, Factor 2, Factor 5, MTHFR, VKORC1 using hydrolysis chemistry, high resolution melt and LC-green designs
• Conducted optimization, development and validation for customer assays utilizing qPCR and high resolution melt genotyping.
• Performed contract research and development with RT-qPCR assays for bladder cancer detection.
• Worked in accordance with cGMP, writing SOP’s and conducting studies for RUO and IVD products
• Performed a real time demonstration of SNP genotyping assays, (consent forms, sample collection, purification, qPCR assay, repeating, auditing and reporting of results).
• Utilized: cGMP/GLP, UV Spectrophotometer, PAGE, Cycle Sequencing, RT-qPCR, qPCR, Long Range PCR, PCR, assay development, multiplexed SNP genotyping, ABI 3130 Sequencer, Agilent 2100 Bioanalyzer, and

Franklin Lakes, New Jersey, US

• Performed development on micro-needle glucose sensors, utilizing a novel glucose detection method, leading to human clinical study
• Worked on SERS (Surface Enhanced Raman Spectroscopy) assays for clinical diagnostics, applying standard assay development to gold nanoparticle signal enhancement technologies
• Regularly summarized progress and presented results at group, and project meetings
• Utilized: cGMP, UV Spectrophotometer, Fluorometer, NHS/EDC chemistry, UV lamp/oven, clean room, Lateral Flow, Nucleic Acid binding and Protein binding Assay design

US

• Worked in cross department meetings, for the development of DEL (DNA Encoded Library).
• Developed and implemented three affinity selection technologies, (Phage Display, mRNA Display, DNA Encoded Library) and improved affinity maturation techniques
• Generated lead candidates for the pharmaceutical pipeline
• Developed and implemented multiple in-house assays to test selected lead candidates
• Regularly summarized progress and presented results at group, and project meetings.
• Utilized: PCR, qPCR, ELISA, FRET, fluorescence, fluorescence polarization, radioactive assay, affinity maturation, affinity purification, size exclusion purification, Biacore (SPR), cloning, viral culture, bacterial.

Boston, MA, US

• Designed, developed and implemented a multiplexed PCR and linear extension, MALDI-TOF based SNP genotyping assay for Cystic Fibrosis, utilizing the Sequenom platform
• Performed three & four primer, multiplexed PCR, linear extension, DNA Purification, SNP genotyping assays, primer/probe design and assay development
• Utilized: Multimek 96, nL dispenser, Bruker MALDI-TOF, and Sequenom genotyping system

• Produced diagnostic ELISA’s with a manufacturing team in accordance with cGMP
• Performed research and development of ELISA assays for cancer diagnostics
• Performed research and development of PCR, RT-PCR, gel, ELISA assays for anemia project
• Evaluated drug candidates for Erythropoietin stimulation in animal and cell models
• Utilized plate washers, ELISA plate coater, RT-PCR, PCR, ELISA, RNA & DNA Purification, PAGE, Western Blot, Cell/Tissue Culture, Transient Transfection and Animal Work (transgenic mice)

• Worked independently, making hybridization mix, performing Southern Blot hybridization, washing and preserving membranes to develop.
• Utilized: hybridization ovens, P32, film cassettes, developer, edge laminator, Geiger counter