Kurt Franzen Email and Phone Number
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Professional Summary: Extensive experience in molecular biology for research & development, and clinical applications including: project management, technical advisor, supervisor, and work in accordance with cGMP.Qualifications:Quality: Experienced working in accordance with, GLP, and GMP, including document editing/writing, of SOPs, protocols, reports, and conducting assay qualification and validation.Selection Technologies: Phage Display, mRNA Display (Profusion), DNA Encoded Library* DEL), affinity selection method developmentNucleic: PCR design/development/validation, RT, PCR, qPCR, RT-qPCR, Multiplex PCR, high resolution melt curve analysis PCR, SNP Genotyping, Sequenom, agarose gel, PAGE, sequencing, capillary electrophoresisProtein: microarray, Lateral Flow, ELISA, colorimetric assay, luminescence assay, fluorescence assay, fluorescence polarization assay, FRET assay, nanoparticle SERS (Surface Enhanced Raman Spectroscopy) assay, Biacore (SPR), SDS-PAGE, MALDI-TOF mass spec., Western Blot, Dot Blot, Biuret, BCAPhysical Testing: Rheometery, Tensile Strength, DurometryPurification: RNA and DNA, Protein purification, viral purification, FPLC, column, PhyNexus tips, bead, magnetic bead, CsCl gradient, Iodixanol gradient, Phenol-Chloroform, precipitation, plaque assay/purification FPLC/column: size exclusion, ion exchange, HIC, affinity (IMAC, SA-biotin, IgG, GSH-GST) Sterile Technique: clean room, laminar flow hood, filtered air hood, dead air hood, bench top sterility. Methods: cloning, bacterial culture, viral culture (M13 phage, AD and AAV), cell and tissue culture, Wave bioreactor, transfection; work with HIV, Hepatitis A, B, C and human fluids.
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Team Manager In Research And DevelopmentExosome Diagnostics Feb 2019 - PresentWaltham, Ma, Us
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ScientistHistogenics Corporation Dec 2017 - Jan 2019Waltham, Ma, Us• Worked independently and collaboratively on analytical development for a cell/tissue therapy project• Collaborated with teams as a scientific advisor, mentor and supervisor• Worked in accordance with GMP, wrote SOPs, protocols, reports, and conducted assay qualification and validation• Utilized: Rheometry, Instron tensile strength, durometry, Spectroscopy, fluorescence, RT, qPCR, ELISA, Lateral Flow, SDS-PAGE, tissue culture, cell culture, NHS, Biuret, BCA, and RNA/DNA purification -
Associate ScientistHistogenics Corporation Mar 2015 - Dec 2017Waltham, Ma, Us
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Contract SpecialistHistogenics Corporation Aug 2014 - Mar 2015Waltham, Ma, Us
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Research ScientistAushon Biosystems Jun 2013 - Jan 2014• Worked collaboratively on projects to improve and develop custom, protein microarray assays.• Utilized: Luminescence, ELISA, 2470 chip arrayer, Cirascan imager
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Technician, Process DevelopmentUniversity Of North Carolina At Chapel Hill Mar 2012 - Jun 2013Chapel Hill, Nc, Us• Supervised and worked collaboratively on projects to improve expression and purification of AD, AAV including the manufacture of custom gene therapies• Utilized: FPLC, qPCR, cloning, CsCl/Iodixanol gradient, tissue culture, transfection, and cGMP -
Sr. Associate Research Scientist Ii, ContractorGrifols Nov 2011 - Feb 2012Sant Cugat Del Vallès, Barcelona, Es• Performed validation study for in-house qPCR, qRT-PCR assays for trace pathogen detection• Utilized: cGMP, qPCR, qRT-PCR, sterile technique, RNA and DNA purification -
Research And Development ManagerCancerguide Diagnostics, Inc. Mar 2010 - Dec 2010• Organized studies involving intellectual property to develop new microarray based diagnostics for chemotherapy selection and clinical study companion diagnostic applications• Coordinated work at three sites, studied and summarized new technologies and new IP• Conducted meetings, presented summaries to management, scientific founders and steering committee• Edited SOP’s and helped initiate the Quality System -
Sr. Staff Scientist, Product DevelopmentParagondx Llc Nov 2007 - Feb 2010Us• Designed, developed, optimized and validated multiple SNP-genotyping qPCR assays for Cytochrome P450 2D6, 2C19, 2C9, Factor 2, Factor 5, MTHFR, VKORC1 using hydrolysis chemistry, high resolution melt and LC-green designs• Conducted optimization, development and validation for customer assays utilizing qPCR and high resolution melt genotyping.• Performed contract research and development with RT-qPCR assays for bladder cancer detection.• Worked in accordance with cGMP, writing SOP’s and conducting studies for RUO and IVD products• Performed a real time demonstration of SNP genotyping assays, (consent forms, sample collection, purification, qPCR assay, repeating, auditing and reporting of results).• Utilized: cGMP/GLP, UV Spectrophotometer, PAGE, Cycle Sequencing, RT-qPCR, qPCR, Long Range PCR, PCR, assay development, multiplexed SNP genotyping, ABI 3130 Sequencer, Agilent 2100 Bioanalyzer, and • qPCR machines: ABI 7500, Roche Lightcycler480, Cepheid SmartCycler® and Stratagene • Regularly summarized progress and presented results at group, and project meetings. -
Contract SpecialistBecton, Dickinson And Company, Bdt Mar 2007 - Nov 2007Franklin Lakes, New Jersey, Us• Performed development on micro-needle glucose sensors, utilizing a novel glucose detection method, leading to human clinical study• Worked on SERS (Surface Enhanced Raman Spectroscopy) assays for clinical diagnostics, applying standard assay development to gold nanoparticle signal enhancement technologies• Regularly summarized progress and presented results at group, and project meetings• Utilized: cGMP, UV Spectrophotometer, Fluorometer, NHS/EDC chemistry, UV lamp/oven, clean room, Lateral Flow, Nucleic Acid binding and Protein binding Assay design -
Principal Research AssociatePraecis Pharmaceuticals Jul 2001 - Aug 2006Us• Worked in cross department meetings, for the development of DEL (DNA Encoded Library). • Developed and implemented three affinity selection technologies, (Phage Display, mRNA Display, DNA Encoded Library) and improved affinity maturation techniques • Generated lead candidates for the pharmaceutical pipeline• Developed and implemented multiple in-house assays to test selected lead candidates• Regularly summarized progress and presented results at group, and project meetings. • Utilized: PCR, qPCR, ELISA, FRET, fluorescence, fluorescence polarization, radioactive assay, affinity maturation, affinity purification, size exclusion purification, Biacore (SPR), cloning, viral culture, bacterial culture, RNA, DNA, Protein, PAGE, Western blot, photoactive and NHS chemistry; robots used: colony picker, liquid handler, plate washer and magnetic bead handler -
Senior Research AssociateBoston University School Of Medicine, Center For Human Genetics Feb 2000 - Jul 2001Boston, Ma, Us• Designed, developed and implemented a multiplexed PCR and linear extension, MALDI-TOF based SNP genotyping assay for Cystic Fibrosis, utilizing the Sequenom platform• Performed three & four primer, multiplexed PCR, linear extension, DNA Purification, SNP genotyping assays, primer/probe design and assay development• Utilized: Multimek 96, nL dispenser, Bruker MALDI-TOF, and Sequenom genotyping system -
Research AssociateOncogene Science Diagnostics Inc. (Osi Pharmaceuticals, Bayer) Sep 1996 - Feb 2000• Produced diagnostic ELISA’s with a manufacturing team in accordance with cGMP• Performed research and development of ELISA assays for cancer diagnostics• Performed research and development of PCR, RT-PCR, gel, ELISA assays for anemia project• Evaluated drug candidates for Erythropoietin stimulation in animal and cell models• Utilized plate washers, ELISA plate coater, RT-PCR, PCR, ELISA, RNA & DNA Purification, PAGE, Western Blot, Cell/Tissue Culture, Transient Transfection and Animal Work (transgenic mice)
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Radiation Lab TechnicianGenetic Design Feb 1996 - Jun 1996• Worked independently, making hybridization mix, performing Southern Blot hybridization, washing and preserving membranes to develop.• Utilized: hybridization ovens, P32, film cassettes, developer, edge laminator, Geiger counter
Kurt Franzen Skills
Kurt Franzen Education Details
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University Of North Carolina At GreensboroDouble Major
Frequently Asked Questions about Kurt Franzen
What company does Kurt Franzen work for?
Kurt Franzen works for Exosome Diagnostics
What is Kurt Franzen's role at the current company?
Kurt Franzen's current role is Team Manager in R&D at Exosome Diagnostics.
What is Kurt Franzen's email address?
Kurt Franzen's email address is ku****@****ail.com
What schools did Kurt Franzen attend?
Kurt Franzen attended University Of North Carolina At Greensboro.
What skills is Kurt Franzen known for?
Kurt Franzen has skills like Assay Development, Elisa, Pcr, Molecular Biology, Qpcr, Dna, Sds Page, Purification, Biotechnology, Western Blotting, Sequencing, Research.
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