Kyle Deans Email & Phone Number

Revere, Ma, Us

Boston, Massachusetts, United States

Performed in vivo lipid nanoparticle biodistribution studies through cross team collaboration to determine the localization effects of RNA cargo on gene editing via intravenous dosing. Utilized IVIS imaging and caliper measurements to quantify tumor burden for CAR-T cell expansion and efficacy studies in multiple mouse models.• Characterized lipid nanoparticle biodistribution and tissue tropism utilizing a novel in-house reporter system.• Achieved organ de-targeting utilizing multiple modalities to reduce off-target editing of the lipid nanoparticle RNA cargo.• Leveraged a CAR-T cell multi-plex editing platform to characterize the efficacy of the edits towards controlling tumor burden in mice for solid and disseminated tumor models.• Analyzed and presented data at department meetings for updates and feedback.

Greater Boston Area

Performed anti-cancer compound efficacy studies utilizing a novel high-throughput device implanted within the tumor micro-environment. Conducted independent in vivo murine experiments utilizing a variety of surgical and administration procedures.• Collected and analyzed investigational drug efficacy data using the microdevice in multiple human cell line subcutaneous tumor murine models.• Gathered and analyzed systemic dosing data of gold standard anti-cancer drugs in patient-derived xenograft mouse models to correlate with microdevice platform data.• Performed subcutaneous injections for tumor establishment and subcutaneous implantation of the device into the tumor tissue.

Ann Arbor, Michigan

Conducted experiments using in vitro testing processes for the development and characterization of polymeric nanoparticles aimed to induce tolerance for autoimmune diseases and allergies. • Performed transcription factor protein assays utilizing a novel lentivirus transfection and luminescent imaging process to characterize cell-nanoparticle interactions when challenged using lipopolysaccharide.• Discovered in vitro transcription factor expression interactions with blank polymeric nanoparticles. • Maintained bone marrow derived macrophage cell lines.

Ann Arbor, Michigan

Investigated vaginal bacterial community interactions using a high-throughput microdroplet bio-reactor platform and cellular/molecular quantification.• Collected and analyzed experimental data to support proof of concept for co-cultivating microbial species within the micro droplet bio-reactor platform.• Created qPCR standard curves for use in quantifying bacterial species populations grown in microbiome co-cultures.

Cincinnati, Ohio

Developed a murine ferric chloride injury induced thrombosis model used in the development of a combination stroke therapy utilizing ultrasonic activated microbubbles and tissue plasminogen activator.• Characterized the relationship between ferric chloride exposure duration on the arterial wall and time to occlusion of the vessel. • Performed murine surgery to access and dissect the carotid artery from the surrounding tissue to measure the resulting arterial flow rate after induced thrombosis.

Grand Rapids, Michigan

• Operated CT and PET scanners for the studies of murine models of multiple myeloma. • Monitored animal behavior post-injection and imaging for any irregular symptoms.

Spencer, Indiana

Supported a quality control team of manufacturing engineers. Executed Installation Qualifications on new equipment, and tested operating parameters of products in conjunction with Product Performance Qualifications. • Resolved a product complaint by troubleshooting, designing, documenting, and implementing a new piece of equipment for production-line use.

Master Of Science - Ms, Biomedical/Medical Engineering

Bachelor Of Science - Bs, Biomedical/Medical Engineering

Bachelor Of Science - Bs, Biomedical/Medical Engineering