Kyle Mcsweeney Email & Phone Number

California, United States

Redwood City, California, US

• Perform small scale and at-scale optimization experiments to improve our gamma delta CAR T cell manufacturing processes
• Work collaboratively with the research team to help determine manufacturability and early process optimization experiments for products moving through the research pipeline
• Write and revise MBRs and SOPs for associated manufacturing processes and essential equipment
• Assist in technology transfer activities to a CMO
• Perform routine flow cytometry work to analyze cells throughout the manufacturing process

Redwood City, California, US

Berkeley, CA, US

• Perform all cell culture work associated with the development of a T cell therapeutic, including T cell isolation, expansion, and cryopreservation, as well as culturing of relevant target cell lines
• Engineer gene edited CAR T cells using CRISPR endonucleases and AAV vectors
• Design and conduct experiments analyzing various functional properties of CAR T cells such as cytotoxicity, cytokine production, and antigen dependent growth
• Optimize existing assays and develop new assays to assess functionality of gene knockouts
• Perform multi-color flow cytometry to asses T cells for memory phenotype, exhaustion markers, etc. using the BD LSRFortessa X-20, FACSDiva, and Flowjo
• Perform high-throughput flow cytometry using the Intellicyt iQue and Forecyte software

Berkeley, CA, US

Santa Monica, California, US

• Performed all tasks associated with the manufacture of commercial product following batch records and SOPs
• Worked as part of a small team to execute GMP runs in close collaboration with Product Sciences and Quality
• Performed operations in ISO 7, ISO 8 and ISO 5 cGMP environments in aseptic conditions to ensure personnel and product safety
• Assisted in the development of standard operating procedures, batch records, deviations and change controls

Waltham, MA, US

• Designed and created mutations in E. coli YoaA to ascertain protein-protein interaction site
• Conducted complementation assays to discern impact of YoaA mutations on DNA repair capabilities
• Performed pull-down assays to elucidate the effect of YoaA mutations on its interaction with the chi subunit of DNA polyermase III

Waltham, MA, US

• Constructed a chromosomal DNA damage reporting system in E. coli
• Applied a yeast two-hybrid system to uncover protein-protein interactions between a conserved region in FOXO1 and a human kidney cDNA library
• Developed a reporter system to screen for small molecule interactions that block the expression of shiga toxins in enterohemorrhagic strains of E. coli

Boston, Massachusetts, US

• Synthesized small molecules for multiple kinase inhibitor projects
• Worked to determine successful reaction conditions
• Improved synthesis pathways for multiple building blocks and final compounds
• Analyzed molecules with NMR and LC/MS

Natick, MA, US

• Designed and ran experiments to precipitate zinc oxide onto textiles
• Conducted antimicrobial tests on experimental textiles with S. aureus
• Investigated chemical decontamination and self-decontamination properties of fabrics precipitated with titanium dioxide
• Analyzed samples using a Scanning Electron Microscope and Energy-dispersive Spectroscopy

Boston, MA, US

• Maintained strict schedules for sleep study participants
• Set-up and ran data recording devices including EEG, EKG, and EOG
• Processed, organized, and analyzed study data

Bachelor Of Science (Bs), Biochemistry

Master’S Degree, Biotechnology

Master Of Business Administration (M.B.A.)