Kyle Paul Smith, Phd Email & Phone Number

Boston, Ma, Us

Cambridge, Ma, Us

Waltham, Massachusetts, United States

• Led internal developability studies of antibody, cytokine, and hetero-oligomeric fusion proteins as a part of research-to-development transition to CMC team.• Generated data which enabled nomination of development candidate in four projects.

Waltham, Massachusetts, United States

• Developed and executed accelerated, plasma, pH, methionine/tryptophan oxidation, manufacturability, and pre-formulation stability studies and platforms.• Tested mutations for reducing aggregation, increasing thermal stability, and increasing glycosylation homogeneity in discovery-stage projects.• Developed DSC, SEC-MALS, RP, HIC, and cIEF methods for characterization.• Trained and managed full-time Northeastern University co-op student for six months.• Managed CRO for HDX structural analysis of multi-domain proteins.• Managed student, full-time direct report or 6 months in biophysical techniques and biotech R&D process.

Waltham, Massachusetts, United States

• Characterized antibody, cytokine, and heterotrimeric fusion proteins for stability, purity, and homogeneity.• Qualified DSF to study thermal stability of proteins with different domains, mutations, and buffers.• Optimized CE-SDS method for quantification of active, product-related impurities.• Won “X-Nihilo” Team Award ($2,500) for implementation of new analytical techniques and contributions to IL-15 project.

Cambridge, Ma

• Developed MST binding assay for small molecule hit validation of enzyme target.• Optimized cIEF for charge profiling of engineered antibody variants and enzyme replacement therapies.• Utilized DSF to study thermal stability of proteins with different mutations, buffers, and concentrations.

Greater Chicago Area

• Led and executed structural and biophysical characterization of the intrinsically mixed-folded, human kinetochore protein (Cdt1) using spectroscopy, scattering, and chromatography.• Established milligram-scale protein purification capabilities to enable protein structural studies in a cell biology lab.• Simulated ensemble conformations of mixed folded/unfolded protein using rigid body modeling.• Trained and mentored part-time student in biophysical techniques and manuscript writing.

Chicago, Illinois

• Developed and optimized protein purification for milligram-scale production of proteins using IMAC, AEX, CEX, and SEC.• Solved crystal structure and generated multi-domain model of a novel mitochondrial GTPase (Miro), with implications in neurological diseases.• Calculated NMR structure of novel mitochondrial-modulating protein (VopE), with implications in V. cholerae infection.• Led meta-analysis to study phosphorylation sites near drug binding sites on proteins in the PDB.

South San Francisco

• Optimized a codon replacement strategy to incorporate unnatural amino acids into phage displayed peptides.• Screened and discovered specific binding peptides for epigenetic drug targets.

Baltimore, Md

• Synthesized substrate analogues for use in drug screening as GCPII inhibitors with implications in cancer and peripheral neuropathy• Optimized whole-cell xc- antiporter inhibition assays for use in high-throughput screening

Cambridge, United Kingdom

• Expressed and produced protein complex mutants with desirable ligand interactions based on crystal structures

Phd In Life Sciences, Structural Biology

Certificate, Leadership Essentials

Certificate, Management For Scientists & Engineers

Bachelor Of Science, Chemical & Biomolecular Engineering