Kyle Caltrider Email & Phone Number

Boulder, Colorado, United States

Longmont, Colorado, United States

Ran the research and development department for a contract manufacturer in the cosmetic and dietary supplement space. Worked on a multitude of products on their path from idea inception to market. Required knowledge of many different domains and collaborated with various departments in coordinating activities in bringing a new product to market.

Longmont, Colorado, United States

Helped design and implement a quality management system for a cGMP compliant facility manufacturing cosmetic and dietary supplement products.

Highlands Ranch, Colorado

As Method Development Department Manager, ran Forensic/Clinical Method Development Department for Chematox Lab, Inc. Specialized duties involved brainstorming and development of toxicological assays for abuse drugs. Coordinated R&D interdepartmental collaboration for validations and production phases. Utilized critical thinking and problem solving abilities to manipulate various chemical extraction techniques and detection systems (mainly UPLC-MS/MS)

As Contracted Boulder Diagnostics/Oxford Immunotec Researcher, performed complex contract work for Boulder Diagnostics and Oxford Immunotec, in conjunction with the Anschutz Medical Campus Infectious Diseases Department for the research and development of novel diagnostic biological-based assays.o Researched and Developed an immunological cell-based assay for the diagnosis of Lyme Disease, including cell purification, culture, handling, and manipulation of prokaryotic and eukaryotic cells.o Influential in method development of this assay, performing background literature research, designing and maintaining SOPs, and training individuals in laboratory techniques. Performed and optimized multiple sandwich ELISA procedures for the measurement of cytokine profiles.o Maintain and troubleshoot lab equipment, including automated cell counters, microscopes, CO2 incubators, BCL-2 biological safety cabinets, Dynex® ELISA processing unit, and more. Conducted SDS-PAGE and western blot analysis of bacterial strains and isolated components.o Engaged in bacterial cell culturing, included cloning and strain expansion. Extracted bacterial lipid components and performed preliminary analysis via thin layer chromatography and focused staining techniques; ran detailed UPLC/MS-MS analysis.

Boulder, Co

As Forensic and Analytical Chemist, led toxicological analysis of biological fluids, utilizing immunological and spectrophotometric techniques. Use broad spectrum of immunoassays for clinical and forensic toxicology purposes. Oversee maintenance and script programming on various Tecan autopipetter units and plate washers for optimization and performance of large ELISA runs. Staff subject-matter expert on set-up/implementation of new ELISA assays, procedures, or equipment. Troubleshoot clinical or forensic ELISA/toxicological screening issues. Specialized research and experiments:o Performed spectrophotometric and ELISA analysis of samples for toxic/illicit compounds.o Conducted liquid-to-liquid and solid phase column chromatography extractions ofpharmaceuticals, illicit drugs, and respective metabolic analytes

Boulder, Co

Performed a broad spectrum of research techniques to elucidate mechanisms and functions of proteins in early human gamete fusion and early embryonic stability. Conducted human and murine cell culturing of oocytes and embryos, including isolation, micromanipulation, incubation, and thawing/cryopreservation of cells. Utilized various vital, chemical, and antibody-based fluorescent stains in the analysis of oocytes and embryos. Employed microscopic techniques, with an emphasis on fluorometric detection, including assays such as FRET using laser scanning confocal microscopy (Zeiss LSM 510). Research culminated in paper published in Reproductive Biomedicine Online, Dec. 2013.