Kyle Hill Email & Phone Number

San Diego, CA, US

San Diego, CA, US

• Contract lead in cell-free biomanufacturing feasibility studies working with biotech startups, established pharmaceuticals, and government agencies to express and purify complex biomolecules
• Led internal development and coordinated with outside vendors to conduct scalability studies around lysate filtration (>100L) leading to tech-transfer
• Established alkaline lysis, chromatography, and formulation methods for plasmid DNA at scale (>100mg)
• Scientist lead in the development of bacterial lysates capable of complex post-translation modifications(glycosylation) of recombinant proteins contributing to a patent filing
• Trained and mentored Scientists and Research Associates in areas of cell-free expression, FPLC, and analytics

• Cell-free protein synthesis (CFPS) feasibility study lead for difficult to express transmembrane proteins using Nanodisc carriers; developed processes to allow scalability of this technology
• Expressed and purified enzyme positive enhancers to expand the capabilities of CFPS
• Developed plasmid DNA alkaline lysis and chromatography methods at the bench scale
• Supported the rapid integration and establishment of a new facility following acquisition of our startup

Columbia, MO, US

• Exploration of the molecular interactions that drive viral infectivity, neurodegenerative diseases, and cancer with an emphasis on the development of small molecule and peptide inhibitors
• Express and purify proteins/enzymes, evaluate enzyme activity and kinetics through the development of novel biochemical techniques (qPCR, MST, EM, etc.), and identify inhibitors that can disrupt normal function
• Determination of protein oligomerization and the impact of inhibitors on protein conformation through negative staining Transmission Electron Microscopy (TEM)

Columbia, MO, US

• Expression and purification of plant pararetroviral proteins from internationally devastating crop pathogens such as Cauliflower Mosaic Virus (CaMV) and Rice Tungro Bacilliform Virus (RTBV) for enzymatic.
• Expression and purification of HIV-1 Integrases from clinical isolates for substrate binding, drug inhibition, and escape mutant studies
• Biochemical characterization of RNA aptamers selected to bind to small molecules and proteins
• Utilize cell culture and molecular techniques in the identification of RNA aptamers which package preferentially into budding viroid and extracellular vesicles

US

• Contribute to the conclusive identification of pathogen infected blood samples
• Uphold the criteria and values set forth by our in-house SOPs and FDA oversight GMP/GLP
• Practice safe laboratory procedures in a clean environment with potentially hazardous samples
• Operate specially designed equipment in all processing steps including Thermocycling, Electrophoresis, Southern Blotting, Hybridization/Immunostaining, Development, and Results Interpretation
• Pursue ongoing training in our Research and Results Interpretation departments

Irvine, CA, US

- Attempted to identify conserved promoter motifs within the Anopheles gambiae genome.- Utilized RNA isolation techniques including 5’RACE Reactions, PCRs, and cloning of samples for sequencing and computational analysis.- Explored and applied a variety of bio-informatics tools in the analysis of DNA promoter regions including Hidden Markov Models and.

Irvine, CA, US

- Created web interface for published Transposable Elements research data using MySQL, HTML, and PHP.- Developed undergraduate research project to expound the significance of transposable elements in the protein coding regions of Oryza sativa (rice).- Used PERL and Python in a UNIX environment to accomplish research related tasks

Doctor Of Philosophy - Phd, Microbiology And Immunology

Bachelor Of Science - Bs, Biological Sciences

High School Diploma