Lance Larrabee Email & Phone Number

San Francisco, CA, US

South San Francisco, California, United States

South San Francisco, California, United States

• Contributed to the advancement of the antibody triage processes via the integration of state-of-the-art in-silico tools for antibody characterization. Utilized 23andMe proprietary antibody datasets to evaluate the.
• Performed in-silico epitope binning using AlphaFold-multimer structural predictions
• Evaluated orthogonal methods to support AlphaFold-multimer in-silico epitope binning conclusions
• Hosted summer intern project to evaluate the capacity of ESM-2 to cluster a large antigen-specific B-cell dataset based on paratope similarity
• Contributed to the generation and evaluation of 23andMe's in-house common light chain mouse

South San Francisco, California, United States

• Advanced 23andMe Therapeutics’ in vivo antibody discovery platform. First, with the integration of the clonepix2 discovery workflow and then to Ag+ single B-cell discovery using the 10X genomics platform. Served as.
• Successfully performed multiple DNA immunizations against highly conserved targets
• Established high-throughput epitope binning method using flow cytometry
• Integrated Sartorius iQue Screener+ into discovery workflow

South San Francisco, CA

• Collaborated with automation engineers to create SOPs/protocols for the Biocel 900. Piloted both 96- and 384-well automated ELISA screening of large hybridoma panels. Served as point of contact for scheduling use and.
• Experience with B-cell isolation/purification from murine bone marrow/spleen
• Explored feasibility of subtractive immunizations for highly conserved targets i.e. GPCRs
• Experienced in the use of Hamilton STAR automation for liquid handling/hit-picking

South San Francisco, CA

• Tasked with expanding the Clonepix2 technology in the Hybridoma group work-flow. Determined appropriate assay conditions for Armenian Hamster fusions: seeding concentration per omnitray, secondary concentration for.
• Super-user of Clonepix2
• Expanded Cell barcoding techniques (FACS Multiplex Assays) to additional commonly used cell lines to decrease plate burden on large panel cell binding assays. Also, evaluated freeze/thaw condition of labeled cells.
• Responsible for in-house Lampire Hamster fusions
• Contributed to comparison studies of new TLR adjuvant cocktail

South San Francisco, CA

• Demonstrated success in planning and performing immunizations, fusions (PEG and Electrofusion), screening, and purification for therapeutic, reagent, and anti-id campaigns. Proficient in the use of a wide range of.
• Proven ability to generate functional candidates utilizing electrofusion or peg technologies
• Responsible for small-scale production and purification of selected clones
• Experience working with several different transgenic animal models for lead generation (Harbour Mouse, AlivaMouse, Omni Rat, and wild-type animals)

Paso Robles, CA

• Weekly perform seven mouse splenocyte fusions with the cancerous cell line SP2/Os. Plate, feed, and evaluate the health of newly created hybridomas. Screen and evaluate hybridomas using ELISA. Select positive clones.
• Performed over 400 mouse fusions
• Planned and developed over 2400 western blots
• Strong sterile techniques