Lane Wilson, Phd Email & Phone Number

Melville, New York, United States

Philadelphia, Pennsylvania, United States

Lead Researcher with the Lysosomal Storage disease team of Dr. Juliette Hordeaux in the James Wilson Laboratory/ Gene Therapy Program at UPenn. Implemented modern CNS-directed AAV gene therapies to evaluate the safety and efficacy of gene delivery in non-clinical and preclinical mouse models of lysosomal storage diseases, and preclinical toxicology and pharmacological studies in non-human primatesDeveloped codon optimized gene therapies for non-clinical and clinical proof of concept… Show more Lead Researcher with the Lysosomal Storage disease team of Dr. Juliette Hordeaux in the James Wilson Laboratory/ Gene Therapy Program at UPenn. Implemented modern CNS-directed AAV gene therapies to evaluate the safety and efficacy of gene delivery in non-clinical and preclinical mouse models of lysosomal storage diseases, and preclinical toxicology and pharmacological studies in non-human primatesDeveloped codon optimized gene therapies for non-clinical and clinical proof of concept studies to assess the histological and behavioral correction via intracerebroventricular injections in the neurodegenerative and lysosomal storage diseases of Batten disease (CLN1) and Niemann Pick type A (NPA) Performed meta-analysis of non-human primate serum and cerebral spinal fluid to identify potential diagnostic biomarkers of neurodegeneration, spinal cord axonopathy and DRG associated toxicity after AAV delivery via intravenous and intra cisterna magna injectionsOptimized and applied improved histological methods for the preparation and assessment of ceroid and lipofuschin autofluorescence storage material, neuroinflammation, and microglial activation in Lysosomal storage diseasesManaged direct and indirect reports in non-clinical and preclinical studies to evaluate gene therapy and biomarker discovery via ISH, IHC, and IF imaging and analysis Show less

Farmington, Connecticut, United States

Glycogen Storage Disease Program, Laboratory of Dr. David Weinstein• Invesigated the pathophysiology of liver glycogen phosphorylase and phosphorylase kinase deficiency and the direct effect on the mechanism of glycogenolysis and presentation of ketotic hypoglycemia in murine models of glycogen storage diseases type VI and IX• Examined the effect of long-term glycogen accumulation in ketotic GSD models and presentation of profibrogenic phenotypes through histological practices •… Show more Glycogen Storage Disease Program, Laboratory of Dr. David Weinstein• Invesigated the pathophysiology of liver glycogen phosphorylase and phosphorylase kinase deficiency and the direct effect on the mechanism of glycogenolysis and presentation of ketotic hypoglycemia in murine models of glycogen storage diseases type VI and IX• Examined the effect of long-term glycogen accumulation in ketotic GSD models and presentation of profibrogenic phenotypes through histological practices • Studying the relationship between altered glucose metabolism, enzyme deficiency and de novo lipogenesis between metabolic abnormalities associated with fibrosis versus pathophysiology of hepatocellular adenoma development in GSD mouse models. Show less

Farmington, Ct

Laboratory of Dr. Caroline DealyThesis: Stimulation of Growth Factor Signaling Mediates Pro-Regenerative Responses in a Murine Digit Amputation Model • Characterized EGFR/ErbB signaling in normal limb development stimulates a pro-regenerative response in proximally amputated murine digits with and without the addition of exogenous EGFR ligands • Established an improved proximal second phalanx amputation model (P2) that mimics the regeneration-incompetent injury of traumatic limb… Show more Laboratory of Dr. Caroline DealyThesis: Stimulation of Growth Factor Signaling Mediates Pro-Regenerative Responses in a Murine Digit Amputation Model • Characterized EGFR/ErbB signaling in normal limb development stimulates a pro-regenerative response in proximally amputated murine digits with and without the addition of exogenous EGFR ligands • Established an improved proximal second phalanx amputation model (P2) that mimics the regeneration-incompetent injury of traumatic limb loss, demonstrating an amputation-responsive progenitor cell population is crucial for a regeneration response. • Developed technical expertise in standard and complex histology practices and brightfield imaging• Project development, manager and training of 8 young scientists with studies awarded by the School of Dental medicine at University of Connecticut. • Independent research led to collaborations of research protocols, technical reports, oral and poster presentations at scientific meetings Show less

Farmington, Connecticut, United States

• Derivation and expansion of single cell pedigrees of primary fetal and adult human intestinal epithelial stem cells to study the pathogenesis of Crohn’s disease in 2D and air-liquid interface (ALI) cell culture models• Independent research on C. difficile toxins A/B and their effects on intestinal epithelial integrity and mucosal barrier protection using 2D and ALI cell culture models • Technical expertise in derivation and expansion of patient-derived intestinal epithelial stem… Show more • Derivation and expansion of single cell pedigrees of primary fetal and adult human intestinal epithelial stem cells to study the pathogenesis of Crohn’s disease in 2D and air-liquid interface (ALI) cell culture models• Independent research on C. difficile toxins A/B and their effects on intestinal epithelial integrity and mucosal barrier protection using 2D and ALI cell culture models • Technical expertise in derivation and expansion of patient-derived intestinal epithelial stem cells• Developed excellent skills and expertise in advanced microscopy, immunohistochemistry, immunocytochemistry and histology practices.• Completion of projects in 2 years resulted in 2 co-authorships in high impact-factor, peer-reviewed journals. Show less

Bethesda, Maryland, United States

Laboratory of Janice Y. Chou, PhD• Constructed multiple plasmids to produce recombinant adeno-associated viruses (rAAV) used for gene therapy in GSD type 1A mice. • Performed independent research on the etiology of fibrogenesis and early onset hepatocellular adenoma formation in gene therapy GSD type 1A mice.• Independently designed transgenic and liver-specific GSD-1a mouse model.• Completion of projects in 2 years resulted in 1 co-authorship in peer-reviewed journal.

North Dartmouth, Massachusetts, United States

Laboratory of Tracie L. Ferreira, PhDAnalysis of a Wnt Clade associated with Orofacial Clefting• Characterized the mRNA localization of the Wnt clade by whole mount in situ hybridization in zebrafish embryos • Conducted plasmid preparation and microinjections in zebrafish embryos

Doctor Of Philosophy - Phd, Developmental Biology

Activities and Societies: 2013-2014 President, Graduate Student Organization My thesis focused on the regenerative incompetent amputation.

Bachelor Of Science - Bs, Biology, General

Activities and Societies: Magna cum laude 2009, President, Pre-medical society Undergraduate Research Assistant 2007-2010