Laura Tuffery Email & Phone Number

New York, New York, United States

New York, Ny, Us

Processing sample for Mass spectrometry analysis to support research groupMaintenance of Mass SpectrometerDevelopment of automation platform

San Francisco, California, Us

As part of a diverse group of life sciences industry professionals, I regularly advise the Nextnet product team and its founders. My advisory work focuses on key product feedback and use-case steering cycles within their team's product development and PMF iterations.

New York, Ny, Us

CAR T cell development for patient targeted therapyCell banking for CAR T cells

My research focused on breast cancer and my aim was to describe and simulate the chemotherapy responses of breast cancer patients.

Dublin, Ie

UPLC testing of glycans present in industrial drugs

Dublin, Dublin, Ie

Investigation of the role of SUMO pathway on HIV-1 promoter reactivation:• Drug testing on 4 suspension cell lines and HeLa LTR using 12 to 96-well plates FACS analysis.• Western blot analysis of SUMOylation.• DNA cloning including DNA extraction, digestion and recombination, bacterial transformation, PCR and Sequencing.

Darmstadt, Hessen, De

MBR Creator in the Downstream Processing group (DSP):• Master Batch Record creation using MES.• Compliance support: Deviation and Investigations using Trackwise®, CCPs and CAPAs.• Operator support and training.• Stock management using SAP and iShop.Process Technician in the Downstream Processing group (DSP):• Work in shifting team requiring daily meeting.• Work in GMP and GLP condition with sterile handling in ISO6 area.• Production of recombinant protein at 15KL scale in GMP conditions.• Technology transfer from laboratory scale to industrial scale.• New equipment set up, validation and qualification.• Work instruction redaction according to GMP guidelines.

Investigation of micro-scale expression of fully human and chimeric recombinant monoclonal antibodies in CHO cells:• Development of small scale expression system optimizing cell growth • Genetic engineering to design expression plasmids including: => cloning => PCR analysis => Site-Directed Mutagenesis (SDM) => Sequencing• Chimeric and fully human recombinant antibody production and characterisation: => Transient expression in CHO cells => Purification => Immunoassay such as ELISA and Western blot.• Set up of ELISA assays for fully human antibody analysis for the company needs • Cell banking

Paris, France, Fr

Purification of Bcl-XL as a positive control for the formation of amyloid aggregate in the cell :• ImpecNano project: post grenelle project based on the study of toxicity of nanoparticules on human cells and the formation of amyloïd aggregates• Set up of purification strategy => Desalting=> Affinity Chromatography=> Size exclusion chromatography=> Ion exchange chromatography=> Mixed mode chromatography => Using AKTA purifier with unicorn software• Protein analysis=> Western blot=> SDS-Page, native and 2D electrophoresis => Protein conformation analysis with pdb viewer.

Doctor Of Philosophy - Phd, System Biology

Master'S Degree, Engineering In Biotechnology