Layla Flack Email & Phone Number

Catonsville, Md

Artemis Federal is a cybersecurity consulting firm providing smart solutions for Federal clients in the Washington D.C. and Baltimore region. There is no simple path where you can say, "We did it, we're 100% secure." A more realistic approach is cyber resiliency, and the ability to prepare for and adapt to changing conditions. Every day our committed teams help clients improve their security posture. Artemis Federal is an SBA 8(a)-certified Woman-Owned Small Business (WOSB), with leadership and consulting support in the following areas:SECURITY ASSESSMENT AND AUTHORIZATION (SA&A)-Lead FISMA independent assessment teams-Quantify how required security controls are implemented, optimized, and producing desired outcomes-Update security posture for IT Systems using annual testing and continuous monitoringINCIDENT RESPONSE-Develop/update cyber incident handling and incident response procedures-Identify and evaluate potential breaches, and ensure losses of PII are properly documented-Develop Tabletop exercises and video storyboardsSOCIAL ENGINEERING-Conduct phishing and vishing exercises to test susceptibility of divulging private information, access to computer systems, and fraudulent actions-Launched agency-wide Training and Awareness campaignsPOLICY-Develop/maintain agency-wide protocols designed to protect both electronic and hard-copy non-public information COMPLIANCE-Facilitated team to conduct 15 onsite certifications and 100 cyclical onsite compliance reviews across the United States annuallyIT INVESTMENTS-Maximized the value of IT investments by enhancing the quality of the business planning-Created and presented cost-benefit analysis (CBAs), proposals, roadmaps, and business recommendations to stakeholders

Gaithersburg, Md

• Led portfolio progression of 9 Cell Line Development campaigns • Generated Cell Line Development reports to support FDA filings of INDs • Developed protocols and utilized FACS for single-cell deposition of antibody-expressing CHO and NS0 cells to achieve >99% probability of clonality• Applied Hamilton robotics, ambr and YSI for screening and passaging cell lines• Accelerated timeline of Cell Line Development process by 25% (4 weeks) and presented results company-wide • Administered access privileges for KIMunity (proprietary SharePoint Enterprise Content and Document Management system) for 200+ employees between Cambridge, UK and Gaithersburg, MD• Aided in the conversion of paper to digital laboratory notebooksPublication: High-throughput screening of antibody-expressing CHO clones using an automated shaken deep-well system, Benjamin Wang 1 , Thomas Albanetti 1 , Guillermo Miro-Quesada 1 , Layla Flack 1 , Lina Li 1 , Judith Klover 1 , Kerri Burson 1 , Krista Evans 1 , William Ivory 2 , Michael Bowen 1 , Ronald Schoner 1 , Pamela Hawley-Nelson 1Biotechnol Prog 2018 11 14;34(6):1460-1471. Epub 2018 Oct 14.Publication: Measuring the aggregation of CHO cells prior to single cell cloning allows a more accurate determination of the probability of clonality, Kerensa J. Klottrup, Guillermo Miro‐Quesada, Layla Flack, Ivan Pereda, Pamela Hawley‐Nelson, Biotechnol Prog 2018 05 17;34(3):593-601. Epub 2017 Jun 17.https://doi.org/10.1002/btpr.2500

Gaithersburg, Md

• Developed 6 cell lines for antibody production, including CHO, NS0 and Biowa cell types• Utilized Clonepix FL to identify high-producing clones and achieve >99% probability of clonality for manufacturing cell lines• Quantitated protein titers using OctetQK, HPLC and flow cytometry• Measured metabolites in fed batch shake flasks using NOVA

Gaithersburg, Md

• Applied molecular biology techniques such as transformation, DNA isolation, PCR and sequencing, site-directed mutagenesis, digestion and ligation• Isolated white blood cells and performed RNA isolation to test population variants• Utilized antibody engineering methods such as affinity maturation to develop phage display libraries• Generated knock-out proteins using mutagenesis to investigate antibody-ligand binding• Applied cell-based assays such as ADCC assays• Performed transient transfection and protein purification• Generated protein crystals to study protein structure and interactions

Gaithersburg, Md

• Established cell-based assays such as plating, efficiency and cell toxicity assays to examine the effect of irradiation on serum• Optimized irradiation conditions of recombinant and plasma-derived human proteins using SDS-PAGE and Western Blot• Determined clotting activity of Factor VIII using MLA Coagulation Analyzer• Quantified recombinant protein MCS-F using ELISA

Washington D.C. Metro Area

• Developed and tested the sensitivity of an ELISA-based biosensor for the detection of Staphylococcal Enterotoxin B• Publication: Dodson JM, Feldstein MJ, Leatzow DM, Flack LK, Golden JP, Ligler FS. Fluidics Cube for Biosensor Miniaturization, Anal Chem. 2001 Aug 1;73 (15):3776-80

Master'S Degree, Biotechnology

Bachelor Of Science (B.S.), Biology/Biological Sciences

Activities and Societies: Phi Sigma Pi Honor Society, FASA (Filipino American Student Association)