Layla Flack Email & Phone Number

Catonsville, MD

Artemis Federal is a cybersecurity consulting firm providing smart solutions for Federal clients in the Washington D.C. and Baltimore region. There is no simple path where you can say, "We did it, we're 100% secure." A more realistic approach is cyber resiliency, and the ability to prepare for and adapt to changing conditions. Every day our committed teams.

Gaithersburg, MD

• Led portfolio progression of 9 Cell Line Development campaigns
• Generated Cell Line Development reports to support FDA filings of INDs
• Developed protocols and utilized FACS for single-cell deposition of antibody-expressing CHO and NS0 cells to achieve >99% probability of clonality
• Applied Hamilton robotics, ambr and YSI for screening and passaging cell lines
• Accelerated timeline of Cell Line Development process by 25% (4 weeks) and presented results company-wide
• Administered access privileges for KIMunity (proprietary SharePoint Enterprise Content and Document Management system) for 200+ employees between Cambridge, UK and Gaithersburg, MD

Gaithersburg, MD

• Developed 6 cell lines for antibody production, including CHO, NS0 and Biowa cell types
• Utilized Clonepix FL to identify high-producing clones and achieve >99% probability of clonality for manufacturing cell lines
• Quantitated protein titers using OctetQK, HPLC and flow cytometry
• Measured metabolites in fed batch shake flasks using NOVA

Gaithersburg, MD

• Applied molecular biology techniques such as transformation, DNA isolation, PCR and sequencing, site-directed mutagenesis, digestion and ligation
• Isolated white blood cells and performed RNA isolation to test population variants
• Utilized antibody engineering methods such as affinity maturation to develop phage display libraries
• Generated knock-out proteins using mutagenesis to investigate antibody-ligand binding
• Applied cell-based assays such as ADCC assays
• Performed transient transfection and protein purification

Gaithersburg, MD

• Established cell-based assays such as plating, efficiency and cell toxicity assays to examine the effect of irradiation on serum
• Optimized irradiation conditions of recombinant and plasma-derived human proteins using SDS-PAGE and Western Blot
• Determined clotting activity of Factor VIII using MLA Coagulation Analyzer
• Quantified recombinant protein MCS-F using ELISA

Washington D.C. Metro Area

• Developed and tested the sensitivity of an ELISA-based biosensor for the detection of Staphylococcal Enterotoxin B
• Publication: Dodson JM, Feldstein MJ, Leatzow DM, Flack LK, Golden JP, Ligler FS. Fluidics Cube for Biosensor Miniaturization, Anal Chem. 2001 Aug 1;73 (15):3776-80

Master'S Degree, Biotechnology

Bachelor Of Science (B.S.), Biology/Biological Sciences

Activities and Societies: Phi Sigma Pi Honor Society, FASA (Filipino American Student Association)