 Developing a bead-based assay aimed at competing with digital PCR, enhancing sensitivity in single DNA molecule detection. Improved the fabrication of a PDMS droplet generator, eliminating the need for chemical surface treatment by introducing a novel method to create hydrophobic surfaces, which simplified the process, making it faster, more reliable, and cost-effective. Identified mRNA biomarkers in Alzheimer’s disease through exosomes in 82 plasma samples, contributing to advancements in neurodegenerative disease research. Built and maintained an ENCODE-standard RNA sequencing data analysis pipeline on AWS, streamlining data processing for in-house experiments. Developed Plot AI, a ChatGPT-integrated tool for data visualization, currently rated 3.7 with over 100K uses and more than 200 user reviews: https://chatgpt.com/g/g-Q7CpjvDRi-plot-ai

 Developed a biofabricated chitosan membrane-based microfluidic platform to study polymicrobial interactions, enabling real-time quantification of signaling and biofilm formation between Pseudomonas aeruginosa and Candida albicans, with potential applications in understanding microbial pathogenesis and human diseases.

 Collected and analyzed wastewater samples from the secondary treatment system to monitor and evaluate system performance, providing critical feedback to operational engineers for decision-making. Successfully handled complex data systems to ensure accurate system evaluations.

 Developed a novel 3D microreactor-based screening assay to study antibiotic responses of surface-independent biofilms, mimicking in vivo conditions. Enabled high-throughput measurements of biofilm tolerance to 1000× MIC antibiotics, advancing the study of antibiotic resistance in tissue-like environments.

Vocation and charity work.

 Developed a microfluidic platform using biofabricated biopolymer membranes to model complex polymicrobial interactions, resulting in NIH grant funding for PhD work (Grant: 1R15GM129766-01). Invented a biofabrication method for creating freestanding biopolymer chitosan membranes with a 100% success rate across 200+ trials, simplifying the membrane formation process in PDMS devices. Managed and constructed out-of-cleanroom photolithography stations for microfluidic device fabrication, optimizing workflow and enabling efficient production in non-laboratory settings.

 Designed, prototyped, and field-tested a pipe-cleaning robot for a fertilizer factory. Led the project’s mechanical design, including gear systems, gear transmission, and shaft calculations, ensuring manufacturing tolerances for milling, lathe, and CNC processes. Collaborated with an electrical engineer to integrate motors and sensors, conducted real-life factory testing, and identified technical improvements for enhanced performance.