Leo Chen Email & Phone Number

San Diego, Ca, Us

San Diego, California, United States

Menlo Park, Ca

Mountain View, Ca

Optimization of media for improved performance of anaerobic Clostridium• Screen different feeds to use as carbon source for solvent production• Optimized media formulation for maximizing titers and yields while minimizing cost• Collaborate on multiple projects for optimal media formulation

South San Francisco, Ca

Engineered bacteria to produce biofuels and chemicals• Performed multiple gene deletions and chromosomal integrations in bacteria to optimize biosynthetic pathway for production of compounds of interest• Generated many gene and plasmid constructs using molecular techniques such as infusion cloning, restriction cloning and megaprimer PCR reaction• Created ribosomal binding site libraries to optimize protein expression• Trained in high throughput machineries• Developed molecular tools and protocol for faster strain engineering• Performed genomic DNA extractions for sequencing to investigate mutations in NTG and transposon strainsEvaluated engineered strains• Performed shake flask and high throughput fermentation in 96 well plate to analyze the performance of strains• Extracted and prepared samples for GC-MS, GC-FID, HPLC analysis

Cloning fungal proteins and expressing in heterologous host. Examine functions and specificities of protein. Purify proteins. Confirm compounds with LCMS and purify with HPLC. Further confirm with NMR (HMQC and HMBC).

M.S., Chemical Engineering

B.S., Chemical Engineering