Capturing flux distribution across metabolic pathways in cannabis chemovars via flux based analysis metabolic modeling.

• Functional Characterization of Soil versus Aquatic Occupying Root Regions in RedAlder via Proteomic Analysis.

Providing administrative support to the Integrative Omics group, such as submitting manuscripts to scientific journals for various scientist, clearing information that is to be made public through PNNL's Information Release system. I am also responsible for helping the Integrative Omics group create and establish a respectable online carbon footprint by managing the groups Twitter handle(@OmicsPNNL).

The aim of our investigation is to assess whether or not the anthocyanin synthesis pathway is involved in the tolerance that some pea lines exhibit towards Fsp. Our approach will be to use the pea line PI271119 as a candidate for our investigation, a highly pigmented variety that has been previously transformed within the Dhingra labororatory. Regeneration and genetic transformation will be completed through CRISPR-Cas9 gene knockout technology in order to mutate Flavanone-3-hydroxylase gene, which is involved in the anthocyanin pathway and can be silenced without affecting the overall development of the pea plant. We will screen the transformed pea plant to ensure accurate transformation and use Sanger Sequencing to search for mutations in the Flavanone-3-hydroxylase gene, in order to evaluate the validity of our hypothesis.