Lexa Staskus Email & Phone Number

• Perform tissue dissociation of mouse spleens and human tumor samples• Culture T cell activations, memory B cell expansions and multiple MACS purifications/isolations• Proficient with multicolor flow cytometry (13 colors) for immune cell phenotyping analysis with FlowJo• Develop FACS strategies with microfluidics devices• Use Octet Detection System for protein quantitation and kinetics• Develop ELISpot protocols for cytokine and antibody secretion analysis• Train scientists on microfluidics instrument• Manage laboratory space in compliance with regulations• Organize laboratory consumables/reagents and maintained laboratory equipment• Develop more environmentally friendly laboratory waste disposal practices

• Performed human iPSC differentiation into oligodendrocytes• Experience with ICC on live and fixed cells followed by analysis using Fiji/ImageJ and CellProfiler• Performed RT-qPCR after isolating RNA and synthesizing cDNA• Experience with human skin samples and cultivating fibroblasts• Sorted mixed neurosphere cultures using fluorescent or magnetic labels

Development of a Blood Brain Barrier Microfluidics Chip and Rabies Virus Glycoprotein Coated Nanoparticles• Perform Neural Stem Cell differentiation into astrocytes• Build microfluidic devices for coculture of two different cell types• Maintain cell lines in culture dishes, cocultured transwells, and microfluidic devices• Perform TEER testing and permeability testing using FITC-Dextran and conjugated liposomes• Present data analysis and write final report

• Performed transfection small scale (200mL) and large scale (2L)• Virus harvest and purification using ion exchange• Tested titer after transduction via PCR and p24 Antigen ELISA assay • Experience with multiple vector digestions and ligations including performing bacterial clone transformations with E. coli