- Designed constructs for protein expression or cell line development.- Expressed, purified, and characterized recombinant proteins and antibodies made from Expi293 and ExpiCHO expression system.- Established human cell lines with lentiviral transduction for antibody screening.- Produced retrovirus and performed retroviral transduction on murine primary B cell.- Worked on platform improvement for HTP monoclonal antibody discovery.- Experienced in hybridoma screening, and single cell sorting.- Characterized therapeutic antibodies using ELISA, multicolor FACS and cell-based assay.- Evaluated and improved lipid based in vitro and in vivo transfection method in human T cell.- Guided two summer interns for the protein expression and purification project.- Generated and characterized nanoparticles using microfluidic system and light scattering system

- Designed constructs for protein expression.- Expressed and purified proteins and antibodies form Expi293 and ExpiCHO cells.- Assisted cytokine level measurement from cell based assay using ELISA.

- Determined the enzyme kinetics parameters of a few hundred plant and microbial proteins for herbicide detoxification.- Identified a few candidates that showed tolerance to a new herbicide for further activity improvement through directed evolution.- Responsible for assay development, protein expression, purification, and enzyme characterization for selected herbicide detoxifying enzymes.- Discovered the promoter region that suppresses the expression of an herbicide detoxifying gene from Arabidopsis using protoplast or tobacco BY-2 system.

- Worked as a key member of the herbicide tolerance research team, responsible for protein purification, experiment design, and partnered with other groups to complete the project assignment.- Collaborated with other companies for the research project. The collaboration project reached its first milestone in 2010.- Completed method development and refinement for various enzyme activity assay, enzyme kinetic characterization and LC-MS analysis.- Worked with team members to complete enzyme activity screening of 1000 enzymes toward detoxification of herbicides.- Prepared documents for paper and patent application regarding the enzymes that can detoxify 2,4-D and dicamba herbicide.- Wrote a research proposal for the identification of enzymes containing new mechanisms for herbicide detoxification.

- Conducted a variety of research in anthocyanin biosynthesis and functional characterization of the transcription factors that control the phenotype.- Designed molecular markers of the candidate genes that control anthocyanin biosynthesis in purple cauliflowers using the sequence information of Arabidopsis, cauliflower and turnip.- Identified the key transcription factor that controls anthocyanin biosynthesis from three family proteins that aligned in tandem via gene mapping using 1900 plants in the mapping population.- Created constructs for plant transformation and yeast two hybrid study.- Successfully generated a construct with a 9kb gene of interest inserted.- Produced thirteen Arabidopsis and four cauliflower transgenic lines for phenotypic complementation and gene functional study.- Collaborated with lab members in characterizing the transcriptional regulation of anthocyanin biosynthesis in four red cabbage cultivars.- Published 3 research articles and made 4 poster presentations in the ASPBmeeting.- Guided 4 undergraduate students in their research projects, and trained studentsto improve their laboratory research skills.

- Responsible for grading, instructing, compiling, and typesetting laboratory materials.

- Generated polyclonal antibodies of papaya allergens from rabbits.- Discovered the papaya and latex cross allergen using Western blot-inhibition and ELISA- inhibition.- Performed protein purification, Western bolt, ELISA, and 1D/2D protein gel electrophoresis.

- Assisted researchers in vascular plants section for field sample collection and preparation of specimen.- Made a series of wooden or paraffin-embedded Myrtaceae specimen for the museum collection.