Key Analytical Skills:• Programming languages: R, Python, SAS, SQL• Bioinformatics Tools/Packages: flowCore, Qiime, ggcyto, DESeq2, edgeR, Seurat, Bioconductor• Development and Version Control: Git, Docker, Conda, Jupyter, Ubuntu, Unix, Linux• Multiomic Analysis: scRNA seq, bulk RNA seq, 16s rRNA seq, flow cytometry • Data Visualization: Seaborn, ggplot2, Shiny, PROC SGPLOT• Data Wrangling: Pandas, dplyr, tidyr. stringr • Statistical Analysis: R (modelr, broom, pwr, survival, XGBoost, caret), SAS (REG, GLM, MIXED, FREQ, TTEST), Python (NumPy, PyTorch, TensorFlow, Keras)• Other: FlowJo, FCS Express, Graphpad Prisim Key Scientific Skills:• Conventional and spectral flow cytometry, cell sorting/FACS and data analysis with FlowJo and FCS Express• ICS and immunophenotyping flow assays• In vitro proliferation assays• PBMC and whole blood processing• ELISA, Legendplex, immunoprecipitation and western blot• DNA/RNA/miRNA extraction, cDNA synthesis, and qPCR• Animal models including EAE, DSS, OVA, HFD, 5-FU mucositis and Tau P301S• Mouse injections/treatments including IP, SC and oral gavage• Mouse behavioral characterization with Morris Water Maze • Mouse breeding/genotyping and colony management• High-throughput RNA-seq and ScRNA-seq library preparation• 16S rRNA seq library preparation• Immunocytochemistry and confocal microscope operations