Liling Shan Email & Phone Number

Utrecht, Netherlands

Utrecht, Netherlands

Department of Pathology and Center for Translational Immunology- Examined proinflammatory role of killer cell proteases in the pathogenesis of rheumatoid arthritis - Investigated whether cytotoxic cells (e.g., CD8+ T and NK cells) can control human cytomegalovirus (HCMV) infection in a noncytotoxic manner besides killing- Examined whether cytotoxic cells can restore cGAS-STING signaling and IFN-β response during HCMV infection- Developed a specific sensor to investigate cytotoxic cell-target cell interactions - Attended national/international conferences and gave 2 oral presentations- Supervised 3 master students from experiments designation to thesis writing- Arranged lab-related duties and ordered laboratory chemicals and reagents timely- Responsible for communication with collaborators regarding experiments designation, data collection and analysis- Published 2 first authorship papers and wrote 4 manuscripts- Techniques used: Cell culture, PBMCs isolation, Cloning, DNA/RNA extraction, (q)PCR, Sequencing, Virus culture, Plaque assay, Viral dissemination assay, Site-directed mutagenesis, Generation of recombinant virus, CRISPR/Cas9, immune cell-mediated killing assay, Immune cell-tumor co-culture, Luciferase reporter assay, Protein/protease expression and purification, Endotoxin assay, Protease activity test, Western Blot, ELISA, Flow Cytometry, Luminex, Confocal microscopy, live cell imaging

Utrecht, Netherlands

Assisted Bachelor Biomedical Research Lab Course- Preliminarily investigated whether NK cells can restore cGAS-STING signaling and IFN-β response during HCMV infection- Responsible for experiments designation and interpretation, coordination tasks between groups, laboratory skills training, trouble-shooting and data analysis- Techniques used: Cell culture, Virus infection, NK cell co-culture with viral-infected cells, Western Blot, Flow Cytometry, ELISA, CRISPR/Cas, RNA extraction, QPCR, confocal microscopy, luciferase reporter assay- The project was selected for oral presentation in Student Research Conference 2018.

Beijing, China

Major in Preventive Veterinary Medicine, research field: Veterinary Microbiology and Molecular Biology- Trained in laboratory animal facility- Investigated interactions between rabbit haemorrhagic disease virus (RHDV) and differentially expressed proteins in the liver- Participated in immunoserology disease diagnosis and antibody development- Techniques used: 2D-DIGE, Mass Spectrometry, Western Blot, Viral RNA Extraction, RT-PCR, Cloning, Sequencing, Recombinant Protein Expression, Real-time PCR, Yeast two-hybrid screen, ELISA

Doctor Of Philosophy - Phd, Infection And Immunity, Life Sciences

Thesis: Noncytotoxic functions of granzymes in inflammation and antiviral immunity

Master'S Degree, Preventive Veterinary

Thesis: The interactions between differentially expressed proteins in rabbit liver and rabbit haemorrhagic disease virus (RHDV)

Bachelor'S Degree, Veterinary Medicine