Lillianne Bartone Email and Phone Number

Conduct molecular assays supporting the research of vector-borne organisms that cause infectious diseases in companion animals and humans. Process bacterial and cell cultures and maintain cell culture collection. Compile, analyze, document, and draw tentative conclusions. Conduct molecular assays supporting the research of vector-borne organisms that cause infectious diseases in companion animals and humans. Process bacterial and cell cultures and maintain cell culture collection. Compile, analyze, document, and draw tentative conclusions from experimental results. Performed DNA extraction, molecular testing (qPCR, dPCR, sequencing prep) for more than 1,400 samples, and presented statistics for 4 major projects to date.

Characterization of a SAS3 homolog in the human pathogenic yeast Cryptococcus neoformans. Assisted in BSL-2 lab performing reverse genetics, DNA cPCR; sgRNA PCR; RT PCR; Touchdown PCR; Agarose Gel Electrophoresis; Gel purification; DNA extraction; DNA cloning; Gene replacement with homologous recombination; Gene replacement with CRISPR-Cas9; Restriction endonuclease assays; Spot Assays. Built virtual plasmids and primers; Electroporation; and Southern hybridization.

Cutaneous microbiome of the Red-Spotted Newt and its role as a defense against fungal pathogens

Assisted banding migrating Northern Saw-whet Owls in order to study their migration dynamics, mating behavior, and how famine affects migration.