Linda Dansereau Email & Phone Number

Sydney, Nsw, Au

Sydney, New South Wales, Australia

Sydney, Australia

Established a C. elegans facility to study the role of mitochondrial drugs on muscle health and longevity. Developed a NAD metabolomics analysis pipeline to detect and quantify components of the NAD metabolic pathway in muscle tissue.

Palo Alto, California

Developed a molecular prototype to study the role of molecular tension during the application of ultrasound to mammalian neurons.

Santa Clara, Ca

Maintained agrobacteria and microalgae coloniesManaged the BioCurious outpost

Sydney, Australia

Activity dependent bulk endocytosis (ADBE) occurs at neuronal synapses during high activity to compensate for large amounts of internal membrane that fuse with the plasma membrane. ADBE is more rapid than constitutive clathrin mediated endocytosis and is triggered by high activity. New synaptic vesicles (SVs) derived from the bulk endosomes (BEs) primarily refill the recyclable pool of vesicles yet the intervening steps from BEs to SVs are unknown. Also unclear is whether internalized BEs are distinct from early endosomes where internalized receptors and cargo are sorted. In order to identify the proteins localised to BEs as a first step towards understanding ADBE regulation I developed a purification method to isolate BEs from SVs for subsequent mass spectrometry (MS) analysis. This involved loading the BEs in active synaptosomes with an uptake marker, isolating intact organelles from the synaptosomes and separating them on a linear density gradient. Using western blot, electron microscopy and mass spectrometry techniques I have identified the fractions which contain BEs.

Sydney, Australia

I performed immunohistochemistry experiments comparing microglial markers in brain tissue. I also developed hippocampal culture and live imaging techniques for other projects in the lab. I also participated in lab discussions, and performed lab duties on a regular basis.

Montreal, Canada Area

Local synthesis of proteins is an important mechanism underlying the synaptic changes implicated in learning and memory but the regulation of the mRNA transport to those sites is not well understood. Based on the results of a proteomics analysis of RNA granules I used members of the DEAD box family of RNA helicases to characterize multiple types of RNA:protein complexes that transport and store mRNA in dendrites. I then showed that distinct types of RNA granules transport different mRNAs and are regulated through distinct signalling pathways, thus leading to specific responses of the cell to various stimuli.

Calgary, Canada Area

I performed experiments using bacterially-expressed proteins to determine regions of interactions to understand the molecular interactions of Cysteine string protein with G proteins.

Montreal, Canada Area

Chemical synthesis of peptides for biological testing

Vancouver, Canada Area

Analysis of vitamin and herbal extracts for quality controls

Toronto, Canada Area

Chemical synthesis of indole derivatives for pharmacological testing

Doctor Of Philosophy (Phd), Neuroscience

Local synthesis of proteins is an important mechanism underlying the synaptic changes implicated in learning and memory but the regulation.

Master'S Degree, Neuroscience

A chaperone protein located on synaptic vesicles, CSP was implicated in regulating synaptic vesicle release yet the mechanism was unknown..

Bachelor Of Science (B.Sc.), Biology And Chemistry

Activities and Societies: Chemistry Co-op, Directed Studies (with Dr. Don Moerman), executive member of the Science Fiction Society.