➣ Optimized pretreatment buffer compositions and experimental conditions for cyclic single molecule fluorescent in situ hybridization (smFISH), improving RNA signal to tissue background ratio.➣ Coordinated with engineering team to minor software and hardware issues.➣ Conducted fresh frozen human/mouse tissue sectioning, fixation, and sample preparation.➣ Lead human brain lipofuscin reduction project and developed LipoClear protocol.➣ Collaborated with UCSF school of Medicine in RNA detection and data analysis of human brain arteriovenous malformation (AVM) disease. ➣ Performed imaging processing with in-house software (Esper Process) after image acquisition with in-house automated spatial omics platform (Rebus Esper).➣ Executed experimental work in laboratory, supported manufacturing process, and worked with commercial team for new products validation studies.

Analyzed of microfluidic produced hydrogel vesicles for artificial antigen-presenting cell applications.➢ Designed wafer by AutoCAD and fabricated microfluidic channel.➢ Developed hydrogel Double Emulsion Droplet (DED) with tunable stiffness and biotinylated fluid membrane.➢ Optimized hydrogel DED generation and validated size, stability, and stiffness characteristic of vesicles.

➢ Conducted Expansion Microscopy to detect microtubules in Hela cell, and modified existing methods of Expansion Microscopy.➢ Prepared experiment buffer, cell culture, and conducted cell fixation, permeabilization, blocking,immunostaining, fabricating poly(sodium-acrylate-co-acrylamide) hydrogel.➢ Developed presentation skills through presenting progress to supervisor and team members.

➢ Provide assistance in the research of characterizing interactions between infrared irradiation to macrophages for potential novel treatment for asthma.➢ Learned the basic experiment skills such as immunostaining, Western blot, PCR, Cell culture, and intravenous.

➢ Provide assistance in the research of nanoparticles combining and apply the technology in local infection control.