Ling-Yu Kuan Email & Phone Number

Responsible for providing excellent customer service while serving the community’s outpatient ambulatory health needs. Offers assistance in the Pharmacy by expeditiously filling the patient’s prescriptions within the guidelines of federal, state, and local legal requirements.

Seattle, Washington

Greater Seattle Area

Clinical microbiology lab

Greater Seattle Area

• Optimized and implemented in-vitro direct and indirect ELISPOT to select tumor associate antigens in designing DNA-based vaccine for breast cancer prevention.• Developed dual color FluoroSPOT to screen tumor associate antigens.• Managed, analyzed data and generated data quality monitoring and scientific reports. • Provided technical support in screening ELISA for selecting tumor associate antigens.• Managed and maintained project related laboratory supplies/equipment.

Seattle

• Utilized multi-parameter flow cytometry for immunephenotying and intracellular cytokines in clinical samples by using BD Aria, BD Fortessa, FlowJo and Coulter Kaluza.• Successfully implemented ELISA protocols from Phase III clinical trial samples.• Designed and executed ELISA operator qualification plans to train other users.• Routinely isolated and cryopreserved PBMC from whole blood from clinical samples.• Performed multiplex chemokine/cytokine analysis using MSD platform.• Performed ELISPOT and T-lymphocyte proliferation assay for clinical samples.

Greater Seattle Area

• Effectively developed and executed cell isolation protocol for human adipose tissues.• Analyzed adipose cell population and PBMC using multi-parameter flow cytometry using BD LSR II and BD Aria.• Designed and executed site-directed mutagenesis in bacterial expression system.• Established stable cell lines with transgenes by lipid-mediated transfection system and isolated microsomes for subsequent biochemical analysis.• Performed multiplex cytokine analysis using Luminx platform.• Routinely genotyped SNPs using Taqman and RT-PCR methods for clinical trials.

Teaching Assistant for Microbiology, Biochemistry and Immunology

Tumor Vaccine Group

• Isolated PBMC from human peripheral blood and leukophoresis samples.• Assisted in development of crypreservation protocol using flow cytometry, T-lymphocyte proliferation assays.• Revised standard operating protocols for ELISA for Phase I clinical vaccine trial.• Maintained various mammalian cell lines and primary cell culture. • Served as the laboratory radiation officer, routine laboratory maintenance and supply ordering.

Doctor Of Pharmacy - Pharmd

Master Of Science, Genetic And Cell Biology

Bs, Medical Technology, Biochemistry