Leading the project development of downstream viral vector, therapeutic protein and plasmid purification processes for biopharmaceutical GMP production. Scaling up and transferring from process development to GMP manufacturing.

Development of downstream processes for cGMP Manufacturing, purification of proteins, discovery to process development, protein modification/conjugation. Ongoing projects in therapeutic protein expression, purification, and characterization using AKTA systems, FPLC, HPLC, reverse phase/ion exchange, and SEC chromatography. SDS-PAGE gel electrophoresis, mass spectrometry, tangential flow filtration equipment, centrifugation equipment, and a variety of separation/analytical techniques. Purification and method development in an industrial setting for a variety of therapeutic protein/antibodies. Verbal and written communication with clients. Maintain quality and detailed laboratory documentation on experimental results and SOP's, work in a collaborative team environment.Working in downstream process development (process optimization and demonstration), writing and reviewing protocols, reports, batch records, SOP, process description, process characterization and tech transfer meeting cGMP. Experienced in column packing and qualification, preparative chromatography (AKTA explorer, Pure, Avant, and Bioprocess Skid), Unicorn, scale-down, scale-up models.

Antibody/Fab expression and purification, antibody engineering, immunotherapy. Protein kinase purification, affinity, SEC, FPLC/HPLC chromatography.Protein/antibody characterization, UV-spectroscopy, gel electrophoresis and mass spectrometry.

Molecular biology and protein Expression using expression systems such as E. coli and Baculovirus.Expression vector design and construction.Target protein purification and characterization.Project management, communication and presentation with client.Molecular modeling, Structure based ligand design and optimization. Client management, communication and progressive presentation.

Active participant and team member in all preclinical stages of drug discovery including hit/lead identification and optimization, pharmacological data evaluation, safety review and candidate selection. Structure based drug design.

Structure based drug design for CNS targets.Protocol development for target protein expression and purification.Method design, optimization for co-crystal formation and structure determination.Structure analysis and molecular modeling for ligand optimization.

Studies on structure/function relationships of aspartic proteases. Contributions to the discovery of FDA approved drug for AIDS therapy.protein chemistry, protein expression, refolding, HPLC, FPLC, gel electrophoresis etc.