Lisa Risen Email & Phone Number

Contract With Ibis Biosciences, Carlsbad, CA

• In vivo selection strain development team in support of a U.S. Defense Threat Reduction Agency (DTRA) grant research program
• Provide support for selection of a genetically engineered bacterial strain capable of screening large libraries of candidate reporter enzymes. This complementation assay will be used to develop an implantable electrode.
• Perform recombineering techniques including P1 phage transductions, competent cell preparations and bacterial electroporation/transformationAssay development team member for a multi-analyte respiratory diagnostic on.
• Performed assay design, optimization and validation on a diagnostic platform incorporating microfluidics and multiplex RT/PCR for detection of viral RNA via oligonucleotide probe hybridization on a microarray. The.
• Conducted on-site personnel training for IRB reviewed clinical studies at the Naval Health Research Center and John Hopkins University School of Medicine.Supported Verification & Validation project teams for bacterial.
• Direct pathogen identification on the IRIDICA platform using human clinical and contrived specimens. Performed sample preparation, nucleic acid amplification, post PCR-purification and analysis by ESI mass spectrometry.

Carlsbad, CA

• Reviewer and editor for pharmaceutical risk management reports submitted to the FDA.
• Recruited and screened healthcare professionals (HCPs) for participation in pharmaceutical risk management studies.

Carlsbad, CA

• Coordinated a respiratory pathogen research study with Johns Hopkins University Medical Center under the guidance of an Institutional Review Board (IRB).
• Managed clinical specimen collection at JHU, specimen database tracking, microbial identification on a prototype high throughput multi-locus base composition analyzer which coupled nucleic acid amplification with ESI.
• Responsible for study materials acquisition and incentive procurement for the participating laboratory and patients.
• Completed NIH PHRP certification (Protecting Human Research Participants)

Carlsbad, CA

• Responsible for high throughput screening for the Ibis Chemistry Department
• Performed all Gram +/- MIC and bacterial transcription/translation screening for the Antimicrobial Project Team on a consulting basis.

Carlsbad, CA

• Responsible for developing and implementing multiple high throughput screening (HTS) assays for the Ibis drug discovery effort
• Performed all MIC screening for the Antimicrobial Project Team.
• Developed HTS coupled bacterial transcription/translation assay for antimicrobial drug discovery.
• Developed and performed an in vitro biological screening assay for compounds that specifically inhibit HCV IRES mediated translation.
• Collaborated with outside university research groups (UCI, Iowa State) for cloning and sequencing of the HCV IRES.
• Responsible for HTS transcription/translation and MIC testing for chemical Structure Activity Relationship (SAR) studies.

San Diego, CA

• Responsible for development of DNA probe diagnostics for direct detection of respiratory pathogens within the Product Development Group. Also responsible for developing genetic testing products for forensic, paternity.
• Performed product design, protocol development and validation, and management of pre-clinical studies.
• Optimized target RNA immobilization, oligonucleotide hybridization kinetics and chemiluminescent detection methods.
• Prepared GMP documentation for manufacturing, quality control and technology transfer.
• Designed single and multi-locus oligonucleotides for RFLP analysis of human genomic DNA.
• Optimized DNA Typing procedures and transferred these synthetic nucleic acid probe (SNAP) methodologies to FBI forensic scientists.

La Jolla, CA

• Responsible for preparation and analysis of environmental samples for trace metal composition.
• Performed acid digestion and organic extractions of soil, water and tissue samples for analysis by atomic absorption spectrophotometry.
• Submitted final data reports to EPA and commercial contracts.