 Established and led Protein/Antibody CRO service team, overseeing recruitment, training, and supervision of junior scientists. Developed protocols/SOPs, engaged in business development, and authored final customer reports. Conducted experimental planning, execution, and data analysis/interpretation, fostering effective communication with cross-functional teams and clients for project success. Optimized expression and purification of engineered recombinant proteins/antibodies in mammalian cells and bacteria, from small to gram-scale production Employed affinity, ion exchange (IEX), and size exclusion (SEC) chromatography for antibody, antigen, and recombinant protein purification, ensuring product quality through characterization techniques like SDS-PAGE, HPLC, and endotoxin assays. Innovated processes and technologies to enhance work efficiency and output, providing expertise to crossfunctional teams. Familiarity with antibody generation utilizing single-B cell screening. Experience using bioinformatics tools such as Pymol for sequence and structure analysis

 Played a pivotal role in the 'Ten Thousand Antibodies Initiative', contributing to the antibody discovery process. Directed the Design Department in optimizing immunogen design for high-quality antibody production based on antibody/protein structural characteristics. Led the Antigen Production/Protein Products Department, optimizing production processes. Established high-throughput rapid plasmid construction process capable of constructing over a dozen different plasmids with the same gene sequence, incorporating various tags/tag combinations. Established high-throughput protein expression and purification methods (prokaryotic), achieving rapid and efficient production. Implemented cost-effective high-throughput mammalian cell expression strategies, enhancing efficiency and cost savings.

Purified the recombinant proteins using ÄKTA FPLC systems with UNICORNDesigned mutagenesis design based on protein 3D structures to determinate functional amino acid(s)

 Led research group and trained the team to design immunogen of Abs, express and purify proteins Built up large scale (up to 20 liter) insect cell expression platform with WAVE to express and purify recombinant proteins Codon optimization, subcloning, and expression recombinant proteins Set up and hand-on experience of large scale protein purification with complex systems/steps (bioreactor (fermenter or wave) + TFF (Tangential Flow Filtration) + FPLC)

 Characterized protein-protein and/or protein/DNA interactions. Expertise truncation and point mutant mutagenesis, protein expression and purification in bacteria, insect cells, and mammalian cells. Expert in operation of AKTA FPLC system to purify proteins Well-trained in techniques in molecular cloning work (made hundreds of DNA constructs); mammalian cell culture and generation of stable cell lines using lentiviral vectors. Trained several research technician and undergraduate students

 Expressed and purified recombinant proteins. Determined protein three-dimensional structures by NMR spectroscopy. Determined protein-ligand 3D structures. Predicted, designed, and determined amino acid interactions of protein-ligand.