Laurensius Kevin Lie Email & Phone Number

Denmark

Cracow, Małopolskie, Poland

• As a fellow within the MSCA-ITN OrganoVIR, I developed and applied patient-derived organoids and engineered cell lines to investigate the cross-tissue and cross-species infectivity of coronaviruses.
• Managed 2 research projects on the development and application of patient-derived organoids and lentivirus-modified cell lines as human coronavirus infection models. The projects were carried out in active.
• Supervised 2 interning Master students intheir theses projects, instructing them on carrying out assays involving human tissue and infectious materials to ensure safety, attention to detail, and adherence to bioethical.
• Successfully established and implemented changes in laboratory standard operations protocol which prevented further incidence of mycoplasma contamination of tissue cultures in the lab, significantly reducing delays.
• Daily management and maintenance of lab operations and inventory, including electronic documentation of data, protocols, and cell bank using combinations of MS Powerpoint, MS Word, MS Excel, and OneNote.

Kobe, Hyogo, Japan

• As a research and educational staff, I collaborated in two projects on the development of a mouse model of brain ischemia (stroke) and on understanding the role of vitamin K in lipid metabolism. I also assisted in.
• Coordinated several interdisciplinary research projects under the guidance of principal investigator and senior scientists, resulting in a conference presentation and a scientific publication.
• Executed in vitro and in vivo assays with minimal supervision, exercising independent judgment in carrying out assays, data analyses, and record keeping under set guidance and standards.
• Taught, trained, and guided students on usage of lab instruments and assay implementations with working communication in both Japanese and English. Assisted principal investigator and senior scientists in mentoring and.
• General laboratory maintenance and management of laboratory operations

Leuven, Belgium

Project: ”Host-virus interaction between Hepatitis C Non-structural Protein 5 and human Raf protein” Roles:- Assisting and performing various molecular biology and biochemical assays, particularly recombinant protein expression assays.- Conducted plasmid-based gene cloning and expression of Hepatitis C virus NS5A, as well as human Raf-1. - Maintenance and.

Leuven, Belgium

- Project: ” Evaluation of early response to imatinib in gastrointestinal stromal tumors (GIST)”- Investigated the effect of acute imatinib administration on NMRI nude mice grafted with human GIST cells - Ex vivo evaluation of the changes in phosphorylation pattern of KIT signalling pathway components in GIST cells in response to acute imatinib.

Doctor Of Philosophy - Phd, Virology

Activities and Societies: Member of: - Marie Skłodowska-Curie Actions - Innovative Training Network (OrganoVIR). - American Society for.

Master Of Science In Biomedical Science, Biomedical Sciences, General, Cum Laude

Master of Biomedical Science, graduated cum laude (July 2015)

Honours In Bachelor Of Science (B.Sc. (Hons)), Microbiology And Immunology, Gpa 3.00

Activities and Societies: Monash University Catholics on Campus Monash University Japanese Study Center - Volunteer Library Assistant.