Logan Trimble Email & Phone Number

San Diego, CA, US

San Diego, California, US

• Built out a process development lab from scratch, including multiple FPLCs, filtration and other lab equipment, general lab supplies, and consumables.
• Developed a platform process to purify AAV9 from crude lysate, including clarification by depth and membrane filtration, ultrafiltration and diafiltration (UF/DF) by tangential flow filtration (TFF), affinity capture.
• Leads downstream group that performs experiments to continue to optimize the process, as well as troubleshoot issues that arise in operation of various unit operations.
• Evaluate new constructs in the platform and adjust operating parameters as needed.
• Built out a GLP pilot lab capable of purifying a 200-L stirred tank bioreactor. Activities included scale determination, equipment procurement, fluid management, and waste consideration.
• Prepared for 200-L GLP productions by drafting, reviewing, and revising batch records, SOPs, and working instructions. Successfully executed on the batch records to purify drug substance and drug product for pilot.

Foster City, CA, US

• Led development of a late-stage monoclonal antibody purification process. Tasks included establishing a development timeline, devising the development strategy for each unit operation, designing and executing.
• Developed various polishing chromatography steps in bind/elute and flowthrough mode, including a cation exchange (CEX) step to clear a product-related impurity and an anion exchange (AEX) step. Activities included.
• Developed and optimized a depth filter-based whole cell harvest for a high cell density GMP antibody process. Optimized the same harvest operation for use in a platform antibody purification process.
• Led successful early-stage viral clearance study campaign, including coordination, preparation, execution, and follow-up. Unit operations studied were Protein A capture, low pH viral inactivation, mixed mode-anion.
• Performed analytics as needed, such as HPLC titer, SEC-HPLC, ELISA, and LabChip CE.
• Generated documentation of all laboratory activities including electronic lab notebook (ELN) and reports.

Somerville, Massachusetts, US

• Designed and executed experiments to improve and further understand a platform downstream process developed to purify lentiviral vector (LVV) produced in a suspension cell culture-based transient transfection process..
• Supported the start-up of in-house pilot-scale capabilities. Performed scale-up experiments and purified LVV at the pilot scale to support scientific investigations of the full-scale process.
• Performed subject-matter expert (SME) duties for technology transfers to external manufacturing partners by generating and reviewing batch records and reports, training operators, acting as person-in-plant (PIP) during.
• Managed a co-op student. Trained the student on the unit operations and associated data analysis, designed experiments, advised the student’s projects, and guided the student’s professional development.

Somerville, Massachusetts, US

• Executed experiments to improve the robustness of the process used to purify suspension-derived LVV.
• Performed routine bench-scale purifications to support upstream process development.
• Assisted in the development of a high-throughput purification method using the JANUS liquid handler.
• Participated in a technology transfer by generating and reviewing batch records and acting as (PIP) for PD activities at external manufacturing facilities.

Somerville, Massachusetts, US

• Produced research-grade LVV on the scale of 2 L to 10 L for in-house research. Production duties consisted of entire upstream and downstream processes, including buffer and media preparation, adherent cell culture and.
• Performed cell-based titer assay and other necessary analytics on all final LVV products manufactured in the group.
• Supported the group as needed with other analytics such as ELISAs and qPCR assays.

Cambridge, MA, US

Project objective: To scale up a Hepatitis A virus production process from roller bottle scale to single-use bioreactor scale, in which mammalian cells are grown on microcarriers.- Growing, feeding, and passaging an attachment mammalian cell line in T-flasks.- Running experiments in spinner flasks, a 1L glass bioreactor, and 3- and 15L disposable.

Lawrence Township, NJ, US

Project objective: To fully characterize a single pass tangential flow filtration (SPTFF) unit for incorporation into the manufacturing process at the Bristol-Myers Squibb Syracuse site, to decrease holding volumes of product.- Determined that feed pressure and feed concentration can control the Volume Concentration Factor (VCF) and volume flux, which are.

Westbrook, Maine, US

Project objective: To develop a product to quantify a specific genus of bacteria in a sample of water- Designed experiments to determine the material and platform geometry most conducive to bacterial growth.- Tested new antibiotics, in a design of experiment setting, to allow growth of the target bacteria but kill other bacteria.

Cambridge, MA, US

Project objective: To determine the tautomeric role of certain metals in the mutagenesis of DNA.- Mixed and autoclaved several types of bacterial media.- Inoculated and infected E. coli cells with M13 bacteriophage in environments including different salts and metals in order to determine how well these viruses grew in each environment.- Centrifuged out.

Tampa, Florida, US

Project objective: To automate the diagnosis of Alzheimer's disease by writing programs able to count and identify neural cells and determine the range of focus of a sample on a microscope slide.- Captured images at varying depths through a sample under a microscope, called an image stack, on which the developers ran programs.- Counted and marked cells in.

Bachelor Of Science, Chemical-Biological Engineering

High School Diploma