Lorene Lee Email & Phone Number

Newark, Ca, Us

-Participated in the drug development process by advancing research for sbRNA candidates as a potential therapeutic for neurodegenerative diseases and preparing data packages for use in patent filing -Independently screened selected sbRNA sequence candidates for advancement to the next stage of the Iris pipeline using a luciferase assay system -Independently characterized and cultured a variety of cells including iPSC neurons, fibroblasts, and primary hepatocyte cells from both mouse and human for use in downstream experiments -Handled and lysed frozen mammalian tissue using GentleMacs and FastPrep for downstream processing-Performed AAV transduction on cell lines to test sbRNA candidate sequences -Tested the efficiency of different AAV viruses using flow cytometry-Independently performed protein assays to quantify biomolecules as a direct result of sbRNA treatment -Prepared RNA samples from for sequencing by extracting and quantifying amounts using NanoDrop, Bioanalyzer, and TapeStation assays -Compiled data from these experiments to draw conclusions and make suggestions on experimental next steps using Microsoft Excel and GraphPad Prism-Independently researched and learned to use new equipment to facilitate data collection -Troubleshot awry results by systematically analyzing the procedure and identifying areas that may have contributed to swaps or mistakes. Proposed and enacted procedural changes to prevent similar mistakes in the future. For non-procedural errors, designed and executed experiments to test reagents. -Semi-automated data analysis procedures by developing robust SOPs and templates for routine experiments-Assisted in lab management by creating a searchable freezer inventory to catalog samples and reagents, organizing the freezer by labeling racks, and regularly taking inventory of chemical reagents-Communicated with external vendors to get recommendations for products best fit for Iris research and provided product feedback

Palo Alto, Ca, Us

-Intern in the Dr. Chuck Chan lab performing research in skeletal stem cells-Used FACS to analyze and sort cell populations-Processed human samples to isolate cell populations-Worked with mice samples to develop animal models of cartilage and bone regeneration-Proficient in Gibson assembly, PCR, bacterial plasmid transformation, mini- and midi-preps, protein purification-Produced lentivirus for use in transduction of skeletal stem cells in order to modulate cell fate-Proficient in histology: pentachrome staining, bone decalcifying and tissue prep, and sectioning using a cryostat-Maintained osteosarcoma cell lines and other mammalian cell lines -Compiled and organized data into posters for presentation-Helped write and revise manuscripts for publication -Drafted IRB and APLAC animal protocols to continue benchwork

-WSI, Shallow Water Lifeguard, CPR/AED/First Aid certified-Managed swim lesson programs and other aquatic programs -Organized and coordinated training and team-building events -Assisted in swim lesson administration by assigning classes, communicating with instructors, students, and parents, and helping instructors where needed-Communicated with patrons in-person, through e-mail, and by phone to address their aquatic concerns -Acted as a liaison between patrons and management -Wrote guides and scripts to train other staff members in swim instruction, customer service, and program administration that resulted in an increase in customer satisfaction-Helped design marketing materials and other visuals for use in public-facing areas-Performed pool maintenance and chemical checks -Taught water aerobic classes

San Leandro, California, Us

-Designed and performed R&D experiments to help develop V.1 of a cell free protein synthesis (CFPS) platform-Performed assay development activities regarding protein quantitation (ex. OneGlo)-Ran the CFPS platform for beta testers, grants, and other projects -Participated in regularly occurring meetings to discuss experimental findings, and occasionally presented new data -Helped perform protein analytical duties -Techniques include SDS-PAGE gels, imaging, electrophoresis, Bradford assays, media preparation, autoclave-Practiced bacterial cell culture techniques using shake flasks-Experienced with bacterial cell lysis using a French cell press-Equipment used includes Echo liquid handlers, Opentron liquid handlers, and Synergy plate readers -Wrote SOPs regarding common day-to-day platform activities -Actively helped develop a platform with activities such as process mapping, architecture design, and process reviews-Wrote extensive write-ups regarding research findings and future next steps-Contacted vendors to procure lab supplies and demos-Highlighted current issues and potential issues regarding platform design, and proposed possible solutions-Analyzed data using Google Suite/Microsoft Office, GraphPad Prism, and compiled data slides for presentation-Experienced with flow chart software LucidChart

-Assisted in research activities by preparing experimental materials and performing experiments regarding oligonucleotide amplification using isothermal amplification systems such as RPA (recombinase polymerase amplification) -Assisted other teams with QC activities on certain lab materials-Assisted on optimization projects regarding amplification on a electric chip surface-Helped in developing and performing an automated chip manufacturing protocol and helped with data collection/tracking-Performed basic molecular biology lab work such as PCR, oligonucleotide purification, electrophoresis (PAGE and Agilent Bioanalyzer electrophoresis), Picogreen oligonucleotide staining and microscopy -Learned how to use proprietary company instruments, platforms, and protocols to perform experiments and gather data -Used Google Suite to organize data and perform basic data analysis -Frequent use of ImageJ software for gel analysis. Other imaging instruments include Typhoon and AlphaImager-Regularly met with management to update project progress and next steps-Used Confluence to keep relevant files and people updated on a project-Occasionally skim journals and literature for relevant articles and protocols -Prepare reagent aliquots in a clean room setting using aseptic technique-Compiled data and charts into presentations-Presented data to company personnel and attended frequent meetings to keep updated with progression of company projects

Menlo Park, California, Us

-Performed experiments in an R&D molecular biology lab (BSL2) in order to develop new products and applications using CRISPR technology. Main focus on planning and executing experiments and assay development for internal projects to get data on product concepts and optimize different processes before transferring to production.-Designed experiments to gather data on unique variables and test conditions; redesigned experiments to account for troubleshooting.-Wet lab skills include aseptic technique, mammalian cell culturing (differential and immortalized cell lines), cell transfections, PCR and genotyping, immunocytochemistry, cell cloning, and cell functionality assays such as ATP production and apoptosis marker assays.-Technology applications include SnapGene, RNAseq with RStudio, inference of CRISPR edits (ICE).-Other duties include compiling data graphs, data analysis, and building presentation slide decks.-Frequently collaborated with other departments such as hardware engineering and sales.-Kept updated with current literature to discuss future directions and applications.-Operational projects include establishing a resource page on Confluence outlining customer projects, internal projects, weekly updates, etc.

Menlo Park, California, Us

-Work in a 5S production setting manufacturing oligonucleotides in a clean room environment. -Responsibilities include practicing aseptic technique, following SOP to troubleshoot and operate machinery and programs for oligo synthesis, and meticulously screen products for quality following production. -Prepared quality control documents for shipping with product. -Took inventory and restocked lab as needed to ensure smooth workflow for following shifts. -Also assisted in training colleagues in areas of production and revising and developing SOPs. -Familiar with Confluence and JIRA systems to maintain a smooth workflow within the factory. -Met with management on a regular basis to discuss and brainstorm process optimization and efficiency solutions to implement in the lab. -Used mass spec to quality assure products and troubleshoot potential reagent/machine issues by identifying trends in failed products.-Identified other failure trends by combing through documentation regarding reagents and machine used.-Projects include working on an occurrence management system using the JIRA ticketing system to identify potential issues in the lab and areas for corrective and preventative actions with help from different teams.

San Jose, California, Us

-Performed basic clinic duties such as patient check-in using EHR-Made phone calls to patients and insurance companies -Assisted medical providers in patient care, such as charting, procedure prep, and procedure assistance.-Acted as a liaison among patients, medical providers, insurances, and different clinics. -Prepared paperwork for patient check-in and provider use, such as worker's compensation documents and insurance audits. -Basic in-house laboratory duties included rapid flu/strep testing, urinalysis testing, and preparation of patient samples for out-of-house laboratory testing.-Acted as a shift lead to update management on key figures and critical events

-Acted in both a customer service and emergency responder capacity to provide constant deck surveillance for patrons in facility-Acted as a liaison between patrons and management to address any customer service needs-Acted immediately and appropriately in emergencies to ensure patron safety -Educated patrons on water safety on a regular basis through water safety lessons and policy enforcement -Engaged in regular training sessions to maintain skills and also lead training modules for other colleagues to optimize their skill sets. -Special projects include developing a teaching manual for a parent-and-tot class detailing various tools, activities, and lesson plans to utilize in class sessions; leading and training junior lifeguard classes, and leading lifeguard training modules.-Took specialized certificated classes such as WSI and shallow water lifeguarding

-Educated patrons of all ages on basic water safety skills and techniques-Taught swim lessons in both private and public lessons-Prepared lesson plans to help address specific class needs and deficiencies -Regularly communicated progress and next steps to both participants and parents

La Jolla, Ca, Us

Biochemistry - Cell Biology Student at UCSDSeptember 2015 - December 2018

La Jolla, Ca, Us

Practiced and performed many lab techniques including protein purification, DNA extraction, and DNA sequencing as part of larger projects. Techniques include plasmid cloning, column prep, size exclusion and ion chromatography, PCR, and gel electrophoresis. Kept a dated and detailed lab notebook as appropriate, and wrote lab reports and presentations.

Assist physical therapist in leading patient exercises and set up modalities such as ultrasound and electrical stimulation.

La Jolla, California, Us

Provided 1-1 and group tutoring to middle-school aged students in the field of mathematics; acted as a teacher's assistant in grading and maintaining smooth classroom flow.

Bachelor Of Science - Bs, Biochemistry And Cellular Biology

Master Of Science - Ms, Biotechnology

Education record