Preclinical in vivo and behavioural investigation of regenerative stem cell therapy for neurodegenerative disorders including narcolepsy, Parkinsons and various dementia types (Agnete Kirkeby group)

Preclinical development and target validation of neurotherapeutics

Thesis: An Emerging Role of CaMKII𝛼 in Narcolepsy Type I – A potential pharmacological target for symptom management. ▹ Investigating a potential novel pharmacological target in Narcolepsy type I using narcolepsy mouse models under supervision of Birgitte Rahbek Kornum and Petrine WellendorphHand-in date: 10.01.2023, prospective defence date: 31.03.2023▹ Responsible for implementation and execution of mouse EEG/EMG including technical setup, in vivo protocols, data management… Show more Thesis: An Emerging Role of CaMKII𝛼 in Narcolepsy Type I – A potential pharmacological target for symptom management. ▹ Investigating a potential novel pharmacological target in Narcolepsy type I using narcolepsy mouse models under supervision of Birgitte Rahbek Kornum and Petrine WellendorphHand-in date: 10.01.2023, prospective defence date: 31.03.2023▹ Responsible for implementation and execution of mouse EEG/EMG including technical setup, in vivo protocols, data management and analysis▹ Responsible for implementation and execution of ex vivo techniques including immunohistochemistry and western blotting ▹ Management of several transgenic mouse breeds including generation of novel transgenic mouse strainsGeneral work:▹ In vivo mouse work: EEG/EMG, continuous activity monitoring, behavioural assays, compound efficacy testing, target validation, transgenic mouse breeding | Ex vivo techniques: IHC, imaging, Western blotting, autoradiography | Data analysis: Prism, VBA and R coding | Illustrator | Teaching and supervision of students | Collaborations with academia (Phosphoproteomics, Robles Lab, LMU, Munich) and industry (IDISCO, Gubra, Denmark)Scientific communication: ▹ Speaker at 6th Nordic Narcolepsy symposium, Oslo, Norway. ▹ Lecturer at Folkeuniversitetet 2020, Copenhagen, Denmark▹ Poster presenter at Neurograd Winter School 2021, Prize winner▹ Speaker at "The world is in you/Verden er i dig"-art exhibition series, 2021, Cinemateket, Copenhagen, Denmark▹ Science commentator on sleep research, videnskab.dk and Den Uafhængige Radio, 2021▹ Speaker at Neurograd Winter School 2022, Prize winner▹ Assistant Consulting Reviewer: BMC Neuroscience Show less

Investigating narcolepsy type I by means of in vivo pharmacology, EEG/EMG recordings and analysis in mice. Responsible for implementing immunohistochemistry and protocol design in the lab.

Thesis: Functional dissociation of rat orbitofrontal cortex subregions in reversal learning▹ Aimed to define the neurocircuitry specific to compulsivity that is an endophenotype seen in psychiatric disorders such as OCD and schizophrenia.The project was conducted under the supervision of Mona El-Sayed Hervig▹ Methods: Touchscreen Serial Reversal learning in rats, stereotaxic intracerebral surgery, Viral and non-viral neuronal tracing, pharmacological manipulations by… Show more Thesis: Functional dissociation of rat orbitofrontal cortex subregions in reversal learning▹ Aimed to define the neurocircuitry specific to compulsivity that is an endophenotype seen in psychiatric disorders such as OCD and schizophrenia.The project was conducted under the supervision of Mona El-Sayed Hervig▹ Methods: Touchscreen Serial Reversal learning in rats, stereotaxic intracerebral surgery, Viral and non-viral neuronal tracing, pharmacological manipulations by microinfusion, immunohistochemistry (fluorescence and chromogenic), microscopy and cell counting using stereology. Show less

I was working in Trevor Robbins Group under the supervision of Mona El-Sayed Hervig. I was primarily working with immunohistochemistry (chromogenic, fluorescence, microscopy) on rat brain slices, brain slicing on cryostat, buffer preparations. I also assisted in the handling and training of rats in reversal learning.

Thesis: The hetereogeneity of the midbrainThis project was conducted under supervision of Mia Apuschkin in the lab of Ulrik Gether▹ Investigated the heterogeneity of dopaminergic system in the ventral midbrain of mice ▹ Applied fluorescence immunohistochemistry (triple staining) and confocal microscopy.▹ Applied ImageJ for cell counting

Communication, Coordination, Teamwork, Salesmanship, Event host and planner and customer data registration

Filing of journals which demonstrates my attention to detail and be in control of several numbersAd hoc jobs; refilling, printing, sending mail

• Responsible for sales• Customer service • Planning of the daily activities and area of responsibilities • Task delegation• Goods reception and handling

• Customer service • Team player• Merchandising• Organisation and coordination of goods