Louise Rants Email & Phone Number

Frederiksberg, Dk

Department Of Biology, Functional Genomics

Extending findings from my PhD project to the human HCT116 cell line, generation of knock-in using the CRISPR/Cas9n technique, conduct a pilot screen with a Selleck collection of 857 bioactive compounds to identify inhibitors of the DNA damage response. Writing articles.

Department Of Biology, Functional Genomics

PhD project title: Regulation of Rad51-mediated homologous recombination by BRCA2, DSS1 and RAD52. Mutations in the breast cancer susceptibility gene BRCA2 are associated with predisposition to breast and ovarian cancers, and loss of BRCA2 function leads to genetic instability. The important role of homologous recombination (HR) is to restore integrity and maintain stability of the genetic material. BRCA2 participates in a DNA repair pathway responsible for repairing double-strand breaks (DSB) by HR. BRCA2 interacts with the recombinase Rad51, and it has been shown that both proteins are essential for HR, DNA repair, and the maintenance of genome stability. I sought to understand the mechanism of BRCA2 in governing the activity of RAD51 and to learn how other recombination-associated proteins such as DSS1 and RAD52 contribute to its regulation. I used the yeast-like fungus Ustilago maydis as an experimental system since it has a well-conserved BRCA2-based recombinational repair system that resembles the one seen in human. The Rad51 ortholog is highly similar to the mammalian protein in structure, and the Rad52 ortholog seems relegated to a less prominent role than in Saccharomyces cerevisiae, similar to what is found in human cells. Key findings from Ustilago was further investigated in the vertebrate DT40 chicken cell line.

Department Of Biology, Functional Genomics

Analyzing the regulation of Rad51-mediated homologous recombination by BRCA2, DSS1 and RAD52. Project planning.

New York, Usa

Gaining knowledge and experience with protein expression, protein purification using sequential chromatography steps and pull-down analyses from E.coli extracts.

Work included basic cell culturing skills.

Gentofte, Dept. 717

Administrative work

Eu-Gmp-Guidelines - Interpretation And Application, Passed The Written Examination

The following chapters were thoroughly reviewed and interpreted – EudraLex Volume 4 Part I, Part III and Annex 1, 8, 9, 15 and 19. Part II.

Doctor Of Philosophy (Ph.D.), Biochemistry

Thesis: "Regulation of Rad51-mediated homologous recombination by BRCA2, DSS1 and RAD52" Supervisor: Professor Michael Lisby, Functional.

Master Of Science (M.Sc.), Biochemistry

Thesis: ”Regulation of homologous recombination by the BRCA2 breast cancer susceptibility gene” Supervisor: Associate Professor Michael.

Bachelor Of Science (B.Sc.), Biochemistry

Thesis: “Analysis of gene expression in normal cells and oncogene-transformed mouse fibroblast cells” Supervisor: Associate Professor.

One Semester (Fall), Undergraduate Studies

Courses attended and passed: Advanced Cell Biology Proteins and Proteonomics Biomembranes Microbial Physiology Virology